We narrowed to 15,898 results for: genome

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pDHt/sk-CE

Plasmid

#92128

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Promoter

Pcbh2

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pB006

Plasmid

#182320

Purpose

It's an empty replication plasmid backbone in Bacteroides

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-GATA6

Plasmid

#232648

Purpose

transient expresses GATA6 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

GATA6 (GATA6 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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SupD v2

Plasmid

#198598

Purpose

Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SupD

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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modRNAc0-p53DD

Plasmid

#176902

Purpose

IVT template plasmid for making p53DD modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

p53DD

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134A

Plasmid

#69276

Purpose

Golden Gate entry vector; 4th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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yTREX

Plasmid

#177306

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-1

Plasmid

#61736

Purpose

Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
tracrRNA
crRNA cassette

Use

CRISPR

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pME-minP:luc2

Plasmid

#138354

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Luciferase

Expression

Mammalian

Promoter

minimal promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA421-Cas9tr

Plasmid

#138709

Purpose

Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

tracrRNA-Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL680

Plasmid

#207513

Purpose

GmM4-SpRY CBE-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-2

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-HA-Nedd8-P2A-Hygro

Plasmid

#124306

Purpose

Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

Nedd8 (NEDD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZS160 CRISPEY HDV-HDV NotI

Plasmid

#114453

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Expression

Yeast

Promoter

ScGAL7p

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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