20,048 results

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Hu

Plasmid

#61242

Purpose

Lentivirus expressing shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Lentiviral

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUCM-CLYBL-hNIL

Plasmid

#105841

Purpose

Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGateway 3XFlag Prickle2 flag tagged

Plasmid

#24645

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Prickle 2 (PRICKLE2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
mOrange-N1

Plasmid

#54499

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFR_HCV_xb

Plasmid

#11510

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-Q63L

Plasmid

#12968

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA constitutively active (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAZeo(-) LAMP2C

Plasmid

#89342

Purpose

Expresses human LAMP-2C in mammalian cells

Depositor

Janice Blum

Article

Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

Insert

Lysosomal-associated membrane protein 2C (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1_blasti

Plasmid

#194067

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lysosomes-20

Plasmid

#55308

Purpose

Localization: Lysosome Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

LAMP1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE2max-∆RNaseH-dualU6

Plasmid

#198735

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassettes

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max∆RNaseH

Use

AAV

Promoter

Cbh

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-mAmetrine1.1

Plasmid

#18084

Purpose

Bacterial expression of mAmetrine (version 1.1) fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine1.1

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
TRUPATH Triple Gai3

Plasmid

#196050

Purpose

Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphai3-RLuc8 (GNAI3 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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