We narrowed to 16,981 results for: bon

1. pcDNA3.1-VcnTS-E1015A

   Plasmid

   #213415

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-VcnTS-E1021A

   Plasmid

   #213416

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1021A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-VcnCS-E1015A

   Plasmid

   #213412

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1015A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-VcnCS-E1021A

   Plasmid

   #213413

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1021A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGA-red-mini

   Plasmid

   #196338

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGA-red-maxi

   Plasmid

   #196337

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGA-blue

   Plasmid

   #196336

   Purpose

   Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRA1SEGFPTcIfm

   Plasmid

   #193777

   Purpose

   Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PR promoter and PLTetO-1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTA1cI-T3

   Plasmid

   #193789

   Purpose

   Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Truncated version of Lambda CI, named T3, where the first two codons are deleted
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO-1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGlomyc-Ovol1

    Plasmid

    #172871

    Purpose

    Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMP71-mGFP-llo190-mc38minor

    Plasmid

    #174604

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28P_dCrk

    Plasmid

    #131145

    Purpose

    Expresses 6XHis::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_B3200

    Plasmid

    #91079

    Purpose

    Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    none (begins with P2A to be fused to TALE-VPR_1)

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_B3400

    Plasmid

    #91080

    Purpose

    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    none (begins with P2A to be fused to TALE-VP64_1)

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOD_A3101

    Plasmid

    #91048

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOD_A3301

    Plasmid

    #91049

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge