Skip to main content
Addgene
Showing: 7521 - 7560 of 10228 results

  13. pMS1088

    Plasmid
    #80159
    Purpose
    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Marcelo Sousa
    Article
    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/
    Insert
    HisSUMO-Cys-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    80159-plasmid-map-sequence-id-150741

    Enlarge

  19. pET14b-mEKLF

    Plasmid
    #67834
    Purpose
    Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution
    Depositor
    James Bieker
    Article
    Armstrong et al Cell. 1998 Oct 2;95(1):93-104.
    Insert
    EKLF (Klf1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Full-length mEKLF is aa 20-376; amino acid 19 is …
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pET28a-FgĂŸF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgĂŸF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgĂŸF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgĂŸF3
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

    Enlarge

  25. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

    Enlarge

  26. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

    Enlarge

  37. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

    Enlarge

  40. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

    Enlarge

Showing: 7521 - 7560 of 10228 results