We narrowed to 14,405 results for: eng
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Plasmid#65042PurposeLentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7DepositorInsertGCaMP6
UseLentiviralTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianPromoterMHCK7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-TORCAR (LAMP1 Human, Synthetic)
TagsCerulean, Full-length LAMP1, and YPetExpressionMammalianPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
TagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKJ1712
Plasmid#21870DepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
v5 Cas9-eVLP
Plasmid#228468PurposeExpresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag(C507V)–Cas9
TagsFLAGExpressionMammalianMutationMMLVgag(C507V)PromoterCMVAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
ExpressionMammalianPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PT3.5/CMV-EGFRvIII
Plasmid#20280DepositorAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTAT-HA
Plasmid#35612DepositorPublicationTypeEmpty backboneTags6xHIS, HA, and TATExpressionBacterialPromoterT7Available sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-sACE2(WT)-Fc(IgG1)
Plasmid#145163PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmIL-6 FL
Plasmid#61286Purposedrives luciferase from Wt mouse IL-6 promoter (full length)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseExpressionMammalianPromoterIL-6 full length promoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1219
Plasmid#61250PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegansDepositorInsertsgRNA(F+E)
UseCRISPRExpressionWormPromoterU6 promoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianPromoterCMVAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by