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Showing: 3781 - 3800 of 177604 results

  1. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139996-plasmid-map-sequence-id-275405

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  2. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    127762-plasmid-map-sequence-id-247554

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  3. pC035 - dLwCas13a-msfGFP

    Plasmid
    #91925
    Purpose
    Expresses catalytically inactive LwCas13a for mammalian RNA targeting
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS-3xHA and NLS-msfGFP
    Expression
    Mammalian
    Mutation
    R488A and R1060A
    Promoter
    EF-1a
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    91925-plasmid-map-sequence-id-228361

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  4. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    112645-plasmid-map-sequence-id-217990

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  5. pLV-EF1-Cre-PGK-Puro

    Plasmid
    #108543
    Purpose
    Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    cre recombinase
    Puromycin
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108543-plasmid-map-sequence-id-208672

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  17. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Publication
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    63726-plasmid-map-sequence-id-438433

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  19. pcDNA3-CaMPARI W391F V398L

    Plasmid
    #60787
    Purpose
    Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator
    Depositor
    Loren Looger, Eric Schreiter
    Publication
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaMPARI W391F V398L
    Tags
    Nuclear Export Signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60787-plasmid-map-sequence-id-198297

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Showing: 3781 - 3800 of 177604 results