We narrowed to 9,202 results for: sacs

1. pFW5000

   Plasmid

   #224214

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLifeAct-miRFP713

   Plasmid

   #136562

   Purpose

   F-actin labeling with a near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LifeAct-miRFP713
   

   Use

   Tags

   miRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-LacI-NLS-VP16

   Plasmid

   #103836

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPhsFlp2::PEST

   Plasmid

   #63171

   Purpose

   Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   hsFlp2::PEST
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG659_lexO-FseI_LexA-TF_LEU2MX

   Plasmid

   #154816

   Purpose

   Inducible expression of FseI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   FseI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-2T-RNAPII-CTD wt #14-HA

   Plasmid

   #160688

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA Polymerase II CTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C terminal domain only

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46612

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-335-5p Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46638

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-92a Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pPROEX Hsp104 (1-575)

    Plasmid

    #1318

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (1-575) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa1-575 of Hsp104

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2R3z-AtU3b-tRNA-ccdB-gRNA

    Plasmid

    #118390

    Purpose

    Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    AtU3b promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH5

    Plasmid

    #52531

    Purpose

    expresses FLP recombinase in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBD-pZ-FLAG

    Plasmid

    #183148

    Purpose

    Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 BD
    

    Use

    Yeast two-hybrid

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p415TEF cyto roGFP2-Grx1(yeast)

    Plasmid

    #65004

    Purpose

    Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1
    

    Use

    Tags

    roGFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-EGFP-KASH

    Plasmid

    #154374

    Purpose

    Vector for Flp-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKMV-VioD

    Plasmid

    #65350

    Purpose

    systhesis of vioD gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFB-Flag-MyoD-MyoD

    Plasmid

    #25992

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD-MyoD homodimer (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

25. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pKMV-VioB

    Plasmid

    #65348

    Purpose

    systhesis of vioB gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSSN6

    Plasmid

    #104340

    Purpose

    Express SSN6

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    SSN6 (CYC8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAT57

    Plasmid

    #204303

    Purpose

    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRG714_lexO-AscI_LexA-TF_URA3MX

    Plasmid

    #154822

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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