We narrowed to 18,867 results for: IRE

1. mEmerald-MCM3-N-22

   Plasmid

   #54166

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

   Insert

   MCM3
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRXIP-Myc-YKT6

   Plasmid

   #116945

   Purpose

   Expressing Myc-YKT6 for mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

   Insert

   Synaptobrevin homolog YKT6 (YKT6 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHA-ΔN331-Kin1B

   Plasmid

   #31603

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   ΔN331 kinesin-1B (Kif5b Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-331

   Promoter

   CMV

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pDB036d

   Plasmid

   #99216

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and PproA

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUE1-TSP-ORF68

   Plasmid

   #162650

   Purpose

   Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsLSm1_J

   Plasmid

   #146676

   Purpose

   Mammalian Expression of HsLSm1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsLSm1 (LSM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV HDM2(NES-)

   Plasmid

   #12087

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   HDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Nuclear-export sequence mutant (NES-): L205A, I20…

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

9. GL2 shRNA

   Plasmid

   #86083

   Purpose

   A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   control shRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH3185

    Plasmid

    #132367

    Purpose

    Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAc5.1B-EGFP-DmGIGYF_AH

    Plasmid

    #148918

    Purpose

    Insect Expression of DmGIGYF

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJM126

    Plasmid

    #49339

    Purpose

    bacterial expression of RFA1, RFA2, and RFA3

    Depositor

    Steven Brill

    Article

    He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

    Inserts

    RFA3 (RFA3 Budding Yeast)
    RFA2 (RFA2 Budding Yeast)
    RFA1 (RFA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pBabe-puro-Smad7-HA

    Plasmid

    #37044

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

    Insert

    Smad7 (SMAD7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-Flag mTOR S2035T R2109A

    Plasmid

    #26606

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T R2109A

    Promoter

    Available since

    Nov. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

15. Pprm1-luciferase; his-3

    Plasmid

    #137728

    Purpose

    Communication-activated luciferase in Neurospora crassa

    Depositor

    Louise Glass

    Article

    Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

    Inserts

    Luciferase
    Pprm-1 Promoter
    

    Use

    Luciferase; Neurospora crassa expression

    Tags

    Expression

    Mutation

    Codon-optimized for N. crassa

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS403red-­luxTA2-­4F

    Plasmid

    #166030

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS405red-­phlTA2-­1E

    Plasmid

    #166029

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGK415-­luxTA2-­4F

    Plasmid

    #165971

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-2T FRB S2035I

    Plasmid

    #26608

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    FRB domain only (aa 2015-2114); S2035I

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1(+)-Rab10-FLAG

    Plasmid

    #135559

    Purpose

    Express mouse Rab10 in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEVOL-MmAcKRS1-PylTUUA

    Plasmid

    #182537

    Purpose

    N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

    Depositor

    Wenshe Liu

    Article

    Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

    Insert

    PylTUUA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProK

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Syn:eGFP-CaM-uTEV1Δ(220-242)

    Plasmid

    #135463

    Purpose

    Neuronal expression of uFLARE protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-CaM-uTEV1Δ
    

    Use

    AAV

    Tags

    eGFP, V5

    Expression

    Mammalian

    Mutation

    S219V mutation improves stability and S153N impro…

    Promoter

    Synapsin

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cdk6

    Plasmid

    #1866

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D224Y

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pDB036a

    Plasmid

    #99215

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK and PproD

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET14b PHAS-I F113A

    Plasmid

    #15680

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I F113A (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F113A mutation.

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

29. mEmerald-MCM6-N-22

    Plasmid

    #54172

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM6
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pAAV.CAG-FLEX.iGABASnFR.F102G

    Plasmid

    #112167

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSicoR (Puro) p65 shRNA2

    Plasmid

    #22508

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA2 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1-GFP-2AFP-KDEL

    Plasmid

    #154349

    Purpose

    Mammalian expression of 2AFP

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2AFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T-3-SH3BP1-CTD

    Plasmid

    #136649

    Purpose

    Expression of SH3BP1-CTD-GST fusion protein in E. coli

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-CTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only C-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX-4T-3-SH3BP1-NTD

    Plasmid

    #136648

    Purpose

    Expression of SH3BP1-NTD-GST fusion protein in E. coli

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-NTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only N-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMito.iGABASnFR.R205A

    Plasmid

    #112178

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R205A (no GABA binding)

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLNHA-C1-HsUPF2-siRNAres_I

    Plasmid

    #146567

    Purpose

    Mammalian Expression of HsUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsUPF2-siRNAres (UPF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET14b_tev_PSGU

    Plasmid

    #59738

    Purpose

    Expresses a.a. 417-887 of human ZO-1

    Depositor

    Alan Fanning

    Article

    Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pVenus-Sec16A

    Plasmid

    #36157

    Purpose

    Expresses Sec16A as a Venus FP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    Venus (EYFP derivative)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

40. FUW-TetO-Gata1

    Plasmid

    #41085

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Gata1 (Gata1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only