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FUGW-PK-hLC3

Plasmid

#61460

Purpose

Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3 (MAP1LC3B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144~451-CMV2-mir1

Plasmid

#164664

Purpose

Express miR-144~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4-P2A-Hygro_Barcode

Plasmid

#170223

Purpose

Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHS2 (hANGPTL4-V5)

Plasmid

#102446

Purpose

Expresses V5-tagged human ANGPTL4 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

ANGPTL4 (ANGPTL4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTBK1

Plasmid

#87443

Purpose

express TBK1 in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

TBK1 (Tbk1 Mouse)

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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peGFP C2-IST1

Plasmid

#218582

Purpose

Expresses GFP-tagged human IST1 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

IST1 (IST1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Cre-p2a-tdTomato

Plasmid

#176837

Purpose

Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Cre-P2A-tdTomato

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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MO91-dCas9-AID(P182X)

Plasmid

#83260

Purpose

Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

Depositor

Xing Chang

Article

Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

Insert

dCas9-AID P182X

Use

Retroviral

Tags

3xFLAG-NLS and FLAG

Expression

Mammalian

Mutation

D10A and H840A in Cas9; P182X in AID

Promoter

MSCV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only
pAAV_CaMKII-TRPV1-flag

Plasmid

#238924

Purpose

Expression of the human TRPV1 channel with a Flag-tag in excitatory neurons

Depositor

Vsevolod Belousov

Article

Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

Insert

CaMKII-TRPV1-flag

Use

AAV

Tags

flag

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Expression

Mammalian

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-SPI1

Plasmid

#97039

Purpose

Expresses SPI1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Spi-1 Proto-Oncogene (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CCNB1-P2A-Hygro_Barcode

Plasmid

#170218

Purpose

Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CCNB1 (CCNB1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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