We narrowed to 28,942 results for: Tat

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-137

Plasmid

#26327

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-137 (Mir137 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga1#3

Plasmid

#122289

Purpose

Knockdown for mouse Hmga1

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112N-pBabe-puro

Plasmid

#19238

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Asparagin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pTY153 EGFP-T2A-ORF6 SARS-CoV-2

Plasmid

#204975

Purpose

Co-express EGFP and SARS-CoV-2 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112H-pBabe-puro

Plasmid

#19237

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Histidin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pTY152 EGFP-T2A-ORF6 SARS-CoV-1

Plasmid

#204974

Purpose

Co-express EGFP and SARS-CoV-1 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-1)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEntry Tshld(FKBP F36V)-GFP

Plasmid

#53260

Purpose

pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld(FKBP F36V)-GFP

Tags

EGFP, Flag, and HA

Mutation

FKBP F36V

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 S245X

Plasmid

#92366

Purpose

expression of M1 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSox10-2

Plasmid

#37007

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSox10-2 (Sox10 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
BAS292 C terminal SF-GFP kanR

Plasmid

#74087

Purpose

S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

SF-GFP recoded for S.pombe

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pEGFPC1/GgVcl 1-851 A50I mutant

Plasmid

#46269

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 A50I mutation (VCL Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain, A50I mutation

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L_R986C

Plasmid

#206176

Purpose

mammalian expression of SAMD9L R986C

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Tags

GFP

Expression

Mammalian

Mutation

R986C

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Mef2D-shRNA

Plasmid

#32900

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2d (Mef2d Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRRG304-Ndk

Plasmid

#99072

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ndk (nou darake) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDIV489

Plasmid

#177697

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL80036

Plasmid

#68259

Purpose

Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRSM17

Plasmid

#101641

Purpose

Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

eqFP486

Tags

6XHis

Expression

Bacterial

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro NM23 H1 WT

Plasmid

#11364

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
TXNIP-py

Plasmid

#200090

Purpose

Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(R163A)-Hygro

Plasmid

#187609

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

Depositor

Robert Sobol

Article

Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

Insert

LivePAR(R163A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

R163A mutation in the WWE Domain

Promoter

EF1A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.mBeta

Plasmid

#24175

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

pIRES.Puro.mBeta (Polb Mouse)

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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HA-VHL L188V-pBabe-puro

Plasmid

#19241

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Leucin 188 to Valin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pBabe-puro

Plasmid

#19235

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Histidin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM12

Plasmid

#101643

Purpose

Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meffCFP

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-FYN-WT-Myc-FLAG

Plasmid

#74510

Purpose

Retroviral expression of FYN

Depositor

Lars Rönnstrand

Article

Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

Insert

FYN (FYN Human)

Use

Retroviral

Tags

FLAG and Myc

Expression

Mammalian

Promoter

unknown

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
BAS287 C terminal E2C hygBR

Plasmid

#74082

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-128

Plasmid

#26308

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-128 (MIR128-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pCR-Blunt II-TOPO-PTPRD

Plasmid

#29705

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Cloning vector

Mutation

none

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-193b

Plasmid

#26318

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-193b (MIR193B Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
HA-VHL 1-115-pBabe-puro

Plasmid

#19243

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 116 - 213 (end)

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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