We narrowed to 27,260 results for: Tat

1. LentiV_Neo_SIK3_CR

   Plasmid

   #108104

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation)

   Promoter

   EFS promoter

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-6xHp

   Plasmid

   #214410

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

   Plasmid

   #205540

   Purpose

   Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   TRE3GS

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ePop

   Plasmid

   #50953

   Purpose

   Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

   Depositor

   Lingchong You

   Article

   Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

   Inserts

   E lysis protein
   LuxI
   LuxR
   

   Use

   Tags

   Expression

   Mutation

   Early stop codon introduced in mutated luxR resul…

   Promoter

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIZ GFP C1

   Plasmid

   #202424

   Purpose

   Encodes GFP alone

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG/ATP8B2-HA

   Plasmid

   #209210

   Purpose

   Mammalian expression of ATP8B2

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP8B2 (ATP8B2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS-CyOFP

   Plasmid

   #74278

   Purpose

   Constitutive expression of CyOFP in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   CyOFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/TO-HOIP-Strep-HA

    Plasmid

    #113494

    Purpose

    Expression of human HOIP with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    HOIP (RNF31 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. VCP(A232E)-EGFP

    Plasmid

    #23973

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    A232E

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. bArr1-V5

    Plasmid

    #201492

    Purpose

    Expression of the beta-arrestin1 containing a C-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr1-V5 (ARRB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO GFP DNAJB1

    Plasmid

    #19495

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mRFP-mem

    Plasmid

    #55622

    Purpose

    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    mRFP-Mem
    

    Use

    Tags

    The N-terminal 20 residues of neuromodulin are fu…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW212

    Plasmid

    #172196

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKII-jGCaMP7f-WPRE

    Plasmid

    #203848

    Purpose

    Expression of GCaMP7f in CaMKII expressing cells

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    jGCaMP7f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-FAB(ER-LD)

    Plasmid

    #214420

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-FLAG-APEX2-LGALS8

    Plasmid

    #175758

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS8 (LGALS8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FgH1tUTG_mup53_Ex5

    Plasmid

    #85535

    Purpose

    Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHY002_Strep_Avi_hsDCAF15

    Plasmid

    #174479

    Purpose

    Expresses DCAF15 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DCAF 15 (DCAF15 Human)
    

    Use

    Tags

    StrepII-Avi-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-sgRb1/Cre

    Plasmid

    #89647

    Purpose

    Expresses an Rb1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rb1 (Rb1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCambia2300 - ProRUBY:RUBY-Citrine

    Plasmid

    #128796

    Purpose

    Fluorescent reporter for subcellular localisation and expression analysis of RUBY

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

    Insert

    RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    ProRUBY

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti4-Puro-H19

    Plasmid

    #200835

    Purpose

    Lentiviral vector expressing human long non-coding RNA H19

    Depositor

    Reinier Boon

    Article

    Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

    Insert

    Human long non-coding RNA H19
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

    Plasmid

    #134337

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1a138

    Plasmid

    #177688

    Purpose

    HCV E1E2 expression plasmid

    Depositor

    Justin Bailey

    Article

    Salas et al Gastroenterology. 2022 Feb;162(2):562-57

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2attB-FST344-HA-CPL

    Plasmid

    #131460

    Purpose

    Encodes human Follistatin protein

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    Follistatin (FST Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pdCas12a(RuvC)

    Plasmid

    #186447

    Purpose

    Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D917A, E1006A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-cofilin WT

    Plasmid

    #78295

    Purpose

    Mammalian expression of mouse cofilin fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pH3R-mCherry-N1

    Plasmid

    #84327

    Purpose

    Histamine-3 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H3R-mCherry (HRH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET11a-AldoA

    Plasmid

    #224925

    Purpose

    Inducible expression of human aldolase A

    Depositor

    Liskin Swint-Kruse

    Article

    Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

    Insert

    Aldolase A (ALDOA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3_NSnoFbox-vhhGFP4

    Plasmid

    #35580

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F box domain of slmb deleted.

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pdCas12b(RuvC)

    Plasmid

    #186450

    Purpose

    Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E848A, D977A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-IB-mRuby3-FTH1

    Plasmid

    #192804

    Purpose

    Stable expression of mRuby3-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO V5 HSPA1L

    Plasmid

    #19511

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 1-like (HSPA1L Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge