We narrowed to 18,334 results for: met

1. Str-STIM1-NN_ST-SBP-EGFP

   Plasmid

   #65262

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet_alphaTubulin_C1

   Plasmid

   #85045

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Alpha-Tubulin (TUBA1B Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-P-CAG-GFPluc2

   Plasmid

   #80493

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   GFPluc2
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. tdTomato-H2B-6

   Plasmid

   #58101

   Purpose

   Localization: Nucleus/Histones, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagRFP-T-Golgi-7

   Plasmid

   #58018

   Purpose

   Localization: Golgi Complex, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET-28c(+)-YTHDF2

   Plasmid

   #102275

   Purpose

   For bacterial expression of His-tagged human YTHDF2

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6X HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-Raichu-RhoA-CR

   Plasmid

   #40258

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Raichu-RhoA-CR (RHOA Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP VAMP8

   Plasmid

   #42311

   Depositor

   Thierry Galli

   Article

   Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

   Insert

   VAMP 8 (VAMP8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Promoter

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEx-4T-1-YTHDF1

    Plasmid

    #70087

    Purpose

    Encode YTHDF1 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast SLC5A7

    Plasmid

    #218539

    Purpose

    human SLC5A7 cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SLC5A7 (SLC5A7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast SLC44A1

    Plasmid

    #218538

    Purpose

    human SLC44A1 cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SLC44A1 (SLC44A1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-KDEL_TNF-SBP-mCherry

    Plasmid

    #65279

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEx-4T-1-YTHDF3

    Plasmid

    #70088

    Purpose

    Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Lenti-sgArid1a/Cre

    Plasmid

    #89642

    Purpose

    Expresses an Arid1a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Arid1a (Arid1a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid

    #189755

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJC6-HK2-3xFLAG

    Plasmid

    #163451

    Purpose

    lentiviral overexpression vector for human HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    HK2 (HK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet_H2A_C1

    Plasmid

    #85051

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmScarlet-i_H2A_C1

    Plasmid

    #85053

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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