We narrowed to 6,546 results for: proc

1. pEX-CFP-STIM1-deltaK

   Plasmid

   #18860

   Depositor

   Tobias Meyer

   Article

   Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Stop codon at amino acid position 671 of STIM1. C…

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-ZIP(RK)

   Plasmid

   #27136

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Arginines in the SH2 domains mutated to lysines (…

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-PIG-Lin28b

   Plasmid

   #64795

   Purpose

   Expresses human Lin28b in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Lin28b (LIN28B Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. G-GECO1.2-Orai1Y80E

   Plasmid

   #73565

   Purpose

   Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   changed tyrosine 80 of Orai1 to glutamate

   Promoter

   CMV Immediate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos3-dnRac1

   Plasmid

   #92040

   Purpose

   Expresses mEos3 fusion with dnRac1 (T17N).

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T17N (dominant negative)

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-H1-shRNA-CMV-HA-PACSIN1

   Plasmid

   #72577

   Purpose

   Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

   Depositor

   Richard Huganir

   Article

   Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

   Inserts

   PACSIN1 (Pacsin1 Mouse)
   H1-PACSIN1 shRNA cassette
   

   Use

   RNAi

   Tags

   HA

   Expression

   Mammalian

   Mutation

   5 silent mutations around shRNA recognition seque…

   Promoter

   CMV and H1

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_SNORD27h

   Plasmid

   #73066

   Purpose

   expression clone for human SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (SNORD27 Human)
   

   Use

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   expression cassette for SNORD27

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. yHS1-M7A,H102A

   Plasmid

   #159152

   Purpose

   Cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   yHS1-M7A,H102A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

    Plasmid

    #199566

    Purpose

    Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Yuxin Song, Andrea Cimarelli

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. Nanog-luciferase targeting construct

    Plasmid

    #26591

    Depositor

    Rudolf Jaenisch

    Article

    Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

    Insert

    Nanog promoter-luciferase-lox-pgk-neomycin-loc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBRPyCAG-fmKlf4-DsRed-Ip

    Plasmid

    #26274

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. psicheck-2-LPPRC-mod

    Plasmid

    #48168

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/T mutation in the ATG of the uORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

17. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG5-FLAG-mEKLF

    Plasmid

    #67833

    Purpose

    Expression of FL-EKLF driven by SV40 promoter

    Depositor

    James Bieker

    Article

    Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    EKLF (Klf1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Full-length mEKLF is aa 20-376; amino acid 19 is …

    Promoter

    SV40

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. J23117-mRFP1-331Bb

    Plasmid

    #78280

    Purpose

    mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23117-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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