159,909 results

1. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124428

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMK

   Plasmid

   #64121

   Purpose

   a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

   Depositor

   Linzhao Cheng

   Article

   Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

   Insert

   MYC-2A-KLF4 (MYC, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMThy1.1_hDDX5_FL

   Plasmid

   #88873

   Purpose

   constitutive expression of DDX5 in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYL156 (TRV RNA2)

   Plasmid

   #148969

   Purpose

   Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

   Insert

   Modified TRV RNA2
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   see comments section below

   Promoter

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-neo-PP1-Alpha

   Plasmid

   #171267

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPXL

    Plasmid

    #12257

    Purpose

    2nd generation lentiviral transfer plasmid

    Depositor

    Didier Trono

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. CaV1.2/pcDNA3.1~ Human

    Plasmid

    #140576

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    human CaV1.2 (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-MCS2

    Plasmid

    #46954

    Purpose

    Cloning plasmid for the generation of Knock-In in human cells using rAAV.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a mCherry

    Plasmid

    #129431

    Purpose

    expression of mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS2513·PHP

    Plasmid

    #122590

    Purpose

    Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

    Depositor

    Pablo Ivan Nikel

    Article

    Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

    Insert

    phIorine2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MBX

    Plasmid

    #64122

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 (BCL-xL) gene

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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