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Rtn2b-mNeon

Plasmid

#186598

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ShhN-Ren

Plasmid

#37677

Depositor

Philip Beachy

Article

Ma et al Cell. 2002 Oct 4;111(1):63-75.

Insert

shh (Shh Mouse)

Tags

renilla luciferase

Expression

Mammalian

Mutation

soluble form (see comments)

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
M-SP-sgRNA

Plasmid

#48671

Purpose

Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJM585 ZF6x6-C mKate2 in TUPV1

Plasmid

#161529

Purpose

Inducible expression of mKate2 under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PB-T3G-ERP2

Plasmid

#175188

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEF SYT1-HaloTag

Plasmid

#197281

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Mutation

N/A

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPD292 ZF43x8-Compact_EYFP

Plasmid

#138903

Purpose

EYFP reporter vector for the ZF43x8-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Compact

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTrans_9xD3B_Cluc_Norm

Plasmid

#68415

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Luciferase

Expression

Mammalian

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pECFP(C1)-PP1alpha

Plasmid

#44233

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pPD057 VP64-ZF37

Plasmid

#138834

Purpose

Constitutive expression of VP64-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Hras

Plasmid

#180573

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPD548 ZF54x6-Compact_EYFP

Plasmid

#138862

Purpose

EYFP reporter vector for the ZF54x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF54x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF54x6-Compact

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTK297

Plasmid

#59566

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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myr-palm_2xmEYFP

Plasmid

#127811

Purpose

Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mEYFP-mEYFP

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pKH3-avian RSK1 K112/464R

Plasmid

#8981

Depositor

John Blenis

Article

Roux et al Mol Cell Biol. 2003 Jul . 23(14):4796-80

Insert

RSK (RPS6KA1 Chicken)

Tags

3x HA

Expression

Mammalian

Mutation

K112R, K464R

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
MTK2_001

Plasmid

#123697

Purpose

Encodes pTRE as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pTRE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSCD-GSDMA-N

Plasmid

#226234

Purpose

Express GSDMA-N domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_FLuc100---MS2

Plasmid

#210574

Purpose

Expresses Optimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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XLone-Puro SARS-CoV2 N P2A eGFP-NLS

Plasmid

#154399

Purpose

PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP

Depositor

Xiaoping Bao

Insert

SARS-CoV2 N Protein (N SARS-CoV2)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV

Plasmid

#62417

Purpose

MXS_chaining vector with CMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN ReP5-GFP-GluR1

Plasmid

#24004

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR1 (Gria1 Rat)

Use

Sindbis virus production

Tags

GFP

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
USP19 sgRNA1

Plasmid

#78585

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA1

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTYF-mCMV/GfaABC1D-EGFP

Plasmid

#19976

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMVGfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pIRESN2 GFP-uvr8at

Plasmid

#44969

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

GFP-UVR8 (UVR8 Mustard Weed, Synthetic)

Tags

GFP

Expression

Mammalian

Mutation

deleted Methionine 1

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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