We narrowed to 21,788 results for: his

1. pTD-C_eYFPTwinStrep

   Plasmid

   #45942

   Purpose

   expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Insert

   synthetic eYFP (codon usage adapted to E.coli K12)
   

   Use

   Tags

   Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq/Ptrc

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Orai2

   Plasmid

   #16369

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai2 (ORAI2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD1028 ZF43x6-Compact(SV40Min)_EYFP

   Plasmid

   #138912

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(SV40min)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF43x6-Compact(SV40min)

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN154

   Plasmid

   #86718

   Purpose

   FLP/Frt dual color reporter for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD1029 ZF43x6-Compact(CMVMin)_EYFP

   Plasmid

   #138913

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(CMVmin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF43x6-Compact(CMVmin)

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEQ1007

   Plasmid

   #83938

   Purpose

   express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

   Insert

   IRS1
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   R833L in IRS1 (please see depositor comment)

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-Rsn2

   Plasmid

   #61686

   Purpose

   bacterial expression of ranaspumin 2 as a His-tagged fusion protein

   Depositor

   Brian Smith

   Article

   Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

   Insert

   Ranaspumin-2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VV001: WT Arch-3 in pET28b

    Plasmid

    #58487

    Purpose

    Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3 (with C-terminal His tag)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD057 VP64-ZF37

    Plasmid

    #138834

    Purpose

    Constitutive expression of VP64-ZF37 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-ZF37
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Parp14-c012

    Plasmid

    #173099

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    W1003-A1190

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid

    #53225

    Purpose

    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

    Depositor

    Jesse Rinehart

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Phosphoprotein synthesis

    Tags

    HIS and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli, both codons for Ser21…

    Promoter

    pLtetO

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Parp14-c010

    Plasmid

    #173100

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    F1208-G1388

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD391 VPR-ZF37

    Plasmid

    #138839

    Purpose

    Constitutive expression of VPR-ZF37 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF37
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD159 dsRedExpress2-ZFi43

    Plasmid

    #138799

    Purpose

    Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    dsRedExpress2-ZFi43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sc eRF1 (WT)

    Plasmid

    #175413

    Purpose

    Encodes His-MBP-TEV-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTDpelB-CTwinStrep

    Plasmid

    #45941

    Purpose

    use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    PelB and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPD535 VP16-ZF129

    Plasmid

    #138768

    Purpose

    Constitutive expression of VP16-ZF129 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF129
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPD303 VP64-ZF43

    Plasmid

    #138835

    Purpose

    Constitutive expression of VP64-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPD158 dsRedExpress2-ZFi37

    Plasmid

    #138797

    Purpose

    Constitutive expression of dsRedExpress2-ZFi37 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    dsRedExpress2-ZFi37
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plIVMD156

    Plasmid

    #160934

    Purpose

    In vivo mRNA display Gateway Destination construct

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPD385 VPR-ZF43(RAAA)

    Plasmid

    #138824

    Purpose

    Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43(RAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge