We narrowed to 10,430 results for: yeast
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Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
ExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
ExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1_K
Plasmid#146740PurposeMammalian Expression of HsNxf-1DepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
Tags3x mycExpressionYeastPromoterADH1Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC1124
Plasmid#41560DepositorInsertUra3-GAL1-UBIYdkGFP*-SpHis5-Tim9
MutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…Available SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS221
Plasmid#34981PurposeExpresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb1–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Ani- α Amylase SP
Plasmid#153503PurposeAspergillus niger α Amylase Signal Peptide Donor PlasmidDepositorInsertα Amylase SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
ExpressionBacterialAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3259 pCMV hUBC9 mt HA
Plasmid#14439DepositorInsertUBC9 C93S (UBE2I Human)
TagsHAExpressionMammalianMutationCys93Ser mutation (TGC changed to AGC)PromoterCMVAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertMitochondrially coded Arg8 protein
MutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12v.3
Plasmid#45121DepositorInsertHtsptLB12v.3
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_AB
Plasmid#98534Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJ1806 pRS413-COF1RFP
Plasmid#37101DepositorInsertCOF1-RFP
TagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …Available SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialPromoterT7-LacOAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan
Plasmid#232099PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg
Plasmid#232098PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC1125
Plasmid#41561DepositorInsertUra3-GAL1-UBIMdkGFP*-SpHis5-Tim9
MutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
Tags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastPromoterGAL1Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
Tags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only