We narrowed to 13,583 results for: TIM

1. pLN343 (FuGW-H2A1p-mKate2 )

   Plasmid

   #105173

   Purpose

   H2A1 promoter drives mKate2 expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   H2A1p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLN381 (FuGW-S(NFY)p-mKate2)

   Plasmid

   #105179

   Purpose

   synthetic promoter NFY drives mKate2 expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(NFY)p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEBB HA BIR 3

   Plasmid

   #25689

   Depositor

   Colin Duckett

   Article

   Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 262-350 (BIR 3)

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pC1300_pUB10_pcoCASphi_E9t_MCS_version2

   Plasmid

   #197950

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PV247

   Plasmid

   #132338

   Purpose

   CasA constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEXP5-NT/pLux LasI

   Plasmid

   #193628

   Purpose

   Inducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LasI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Lux promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLN593 (FuGW-S(MAFK)p-mKate2)

   Plasmid

   #105205

   Purpose

   synthetic promoter MAFK drives mKate2 expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(MAFK)p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-L5-oPRE-L2

   Plasmid

   #32414

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   oPRE
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   WPRE with deletion of X protein promoter and muta…

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

   Plasmid

   #205275

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   E498R

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

    Plasmid

    #205276

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    T513K

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

    Plasmid

    #205274

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D487K

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

    Plasmid

    #205272

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D300R

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEV_S

    Plasmid

    #100266

    Purpose

    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli

    Depositor

    Timothy Lu

    Article

    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.

    Inserts

    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    

    Use

    Synthetic Biology; Expression, pbr322

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCHA1.1-Rap2a-V5

    Plasmid

    #149509

    Purpose

    Stable expression of human Rap2a.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    RAP2 (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMLS544

    Plasmid

    #188882

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKDC.015

    Plasmid

    #184992

    Purpose

    Express -Eco2 RT and ncRNA in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(wt), a1/a2 length: 13
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p1148-pCSDest-AcrIIA4-mCherry

    Plasmid

    #126204

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Use

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEBB HA XIAP H467A

    Plasmid

    #25693

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H467A

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

19. a5-ecto-pcDNA31hygro

    Plasmid

    #113386

    Purpose

    Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human alpha 5 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBB HA BIR 2-3

    Plasmid

    #25691

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acid 124RDH-LEE350 (BIR 2&3)

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PP363

    Plasmid

    #78984

    Purpose

    255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    rHGH
    IFNa2b
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDF-CcAvs4

    Plasmid

    #188808

    Purpose

    Codon optimized CcAvs4 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    KpAvs4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDF-EcAvs4

    Plasmid

    #188806

    Purpose

    Codon optimized EcAvs4 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMLS714

    Plasmid

    #188886

    Purpose

    MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FUp95bGW (B6)

    Plasmid

    #74015

    Purpose

    lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 beta isoform
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant Psd95

    Promoter

    hUbC

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pDD375

    Plasmid

    #91825

    Purpose

    Codon-optimized mKate2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mKate2-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SFG.CNa22_opt.IRES.eGFP

    Plasmid

    #22491

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Sep 21. ():.

    Insert

    Codon optimised Calcineurin A mutant 22 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    V314R; Y341F

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK_kanR_proJK1_PAD

    Plasmid

    #60630

    Purpose

    Constitutively expresses PAD (phenolic acid decarboxylase)

    Depositor

    Timothy Whitehead

    Article

    Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

    Insert

    Phenolic acid decarboxylase padC (padC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K146Y

    Promoter

    proJK1

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FH95pUGW (B3)

    Plasmid

    #74012

    Purpose

    lentiviral expression of EGFP and Psd95 shRNA

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pVP65KR-gb-SacB(-)

    Plasmid

    #171646

    Purpose

    Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.

    Depositor

    Young Kee Chae

    Article

    Chae et al Protein Pept Lett. 2021;28(12):1398-1407

    Insert

    MBP-mCherry-8xHis-SacB(-)
    

    Use

    Tags

    8xHis

    Expression

    Mutation

    internal HindIII site in the SacB gene was remove…

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEBB FLAG XIAP D148A/W310A

    Plasmid

    #25685

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D148A W310A

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pFTK054

    Plasmid

    #171326

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    QS (QA-1S) codon optimized, quinic acid repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEBB HA XIAP delta BIR

    Plasmid

    #25686

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta BIR Contains only aa 331-497

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pEBB XIAP H467A

    Plasmid

    #25680

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H467A

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pEF1-puro/hpb1(short)

    Plasmid

    #59973

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 (short) (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pJAID6A

    Plasmid

    #165046

    Purpose

    Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. a4 ecto pcDNA3.1 hygro(-)

    Plasmid

    #113406

    Purpose

    expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

    Insert

    human alpha 4 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEBB XIAP Delta RING

    Plasmid

    #40828

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta RING: contains aa 1-448

    Promoter

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pEBB XIAP D148A/W310A

    Plasmid

    #25678

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D148A W310A

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

41. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. BlatCas9

    Plasmid

    #163792

    Purpose

    The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pfMDH_H303A

    Plasmid

    #67730

    Purpose

    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pfMDH_D230A

    Plasmid

    #67725

    Purpose

    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed aspartate 230 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pfMDH_N191A

    Plasmid

    #67724

    Purpose

    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 191 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pfMDH_K295A

    Plasmid

    #67726

    Purpose

    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 295 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

47. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. FH95pUP95PDZGW (C6)

    Plasmid

    #74022

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    PDZ

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

49. pJK_proK6_eGFP

    Plasmid

    #59850

    Purpose

    Constitutively expresses eGFP with proK6 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK6

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAWp7-10

    Plasmid

    #72742

    Purpose

    pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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