We narrowed to 27,260 results for: Tat

1. TALED_Left-ND6-1397N

   Plasmid

   #212952

   Purpose

   Expresses TALED_Left-ND6-1397N in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

   Insert

   TALED_Left-ND6-1397N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TALED_Right-ND6-1397C-AD

   Plasmid

   #212956

   Purpose

   Expresses TALED_Right-ND6-1397C-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

   Insert

   TALED_Right-ND6-1397C-AD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM4788

   Plasmid

   #120089

   Purpose

   Cyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.

   Depositor

   Susan Golden

   Article

   Chen et al Microbiology. 2016 Dec;162(12):2029-2041

   Insert

   pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin binding domain of Ezrin

   Plasmid

   #62381

   Purpose

   Expresses the actin binding domain of ezrin. Amino acids 552-586.

   Depositor

   Guillaume Charras

   Article

   Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

   Insert

   Actin binding domain of ezrin (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains aa's 552-586

   Promoter

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO V5 DNAJB8

   Plasmid

   #19531

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRGEB-Cas9-VirD2 (PCO)

   Plasmid

   #139802

   Purpose

   Expressing Cas9-VirD fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   Cas9-VirD2
   

   Use

   Tags

   Cas9-VirD2

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmIL-6 mut C/EBP

   Plasmid

   #61291

   Purpose

   drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

   Depositor

   Gail Bishop

   Article

   Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

   Insert

   IL-6 promoter (Il6 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutated C/EBP binding site from ACATTGTGCAATCT to…

   Promoter

   IL-6 promoter with mutant C/EBP binding site

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

10. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194283

    Purpose

    EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO V5 DNAJB7

    Plasmid

    #19530

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-ETV6-ETS

    Plasmid

    #111180

    Purpose

    Minimal ETS domain of human ETV6 (TEL)

    Depositor

    Gregory Poon

    Article

    Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

    Insert

    ETV6 ETS domain (ETV6 Bovine, Human, Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid

    #133779

    Purpose

    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    Isocitrate dehydrogenase 1 (IDH1 Human)
    

    Use

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Arginine is mutated to histidine at amino acid po…

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ad-EFNA1

    Plasmid

    #102724

    Purpose

    Expression of EphrinA1

    Depositor

    Jin Chen

    Article

    Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

    Insert

    EFNA1 (EFNA1 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-SVIP-Strep-HA

    Plasmid

    #113491

    Purpose

    Expression of human SVIP with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    SVIP (SVIP Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO GFP DNAJB4

    Plasmid

    #19499

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIF1C-mCherry-FRB

    Plasmid

    #185982

    Purpose

    Mammalian expression of human KIF1C with mCherry and FRB tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    mCherry and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-ERBB4 (kinase dead)

    Plasmid

    #29533

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Promoter

    Available since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pAAV-hSyn-Flex-mRuby3-2A

    Plasmid

    #203846

    Purpose

    Neuron specific expression of a Cre-dependent mRuby3

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

    Plasmid

    #123360

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Empty LbCpf1 crRNA cloning cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLIK 3xFLAG-KIF20A zeo

    Plasmid

    #192322

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-KIF20A

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5FRT/TO-RNF185-GFP

    Plasmid

    #113498

    Purpose

    Expression of human RNF185 with C-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    RNF185 (RNF185 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SBP-ST*-GFP

    Plasmid

    #163668

    Purpose

    Expresses SBP tagged partial length ST in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    

    Use

    Tags

    GFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    This chimera contains the first 113 amino acids o…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Venus-7aa-ActA-pEGFP-C1

    Plasmid

    #177405

    Purpose

    To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

    Depositor

    David Andrews

    Article

    Osterlund et al Protocol Exchange (2022)

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pMyc4ElbLuc.

    Plasmid

    #53246

    Purpose

    reporter plasmid containing 4 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc4ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pPB-hCMV1-hNANOG-IV

    Plasmid

    #60432

    Purpose

    tet-inducible human NANOG

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    NANOG (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5/FRT/TO GFP DNAJA3

    Plasmid

    #19493

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA3 (DNAJA3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV Reep3-V5

    Plasmid

    #175119

    Purpose

    Lentiviral expression of mouse Reep3-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Reep3 (Reep3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe puro H-Ras G12V Y40C

    Plasmid

    #12276

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

33. 5K-tails-->R GLUT1-GFP

    Plasmid

    #200099

    Purpose

    human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 451, 456,…

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #191397

    Purpose

    sgRNA targeting alpha-satellites on human chromosome 9

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    sgRNA targeting alpha satellites on human chromosome 9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HES7-NLuc-2A-tdTomato

    Plasmid

    #130932

    Purpose

    Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7 (HES7 Human)
    

    Use

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. XE124 XDsh delta DEP-GFP-CS2+

    Plasmid

    #16785

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

    Insert

    Xdsh-delta-DEP (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    contains Dsh amino acids 1-433 and 503-736 (delet…

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. V5-bArr1

    Plasmid

    #201491

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr1 (ARRB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GST-FBL

    Plasmid

    #179245

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    FBL (FBL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP-C1-BBS4

    Plasmid

    #218713

    Purpose

    Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS4 (BBS4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I354T (t1110c)

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge