We narrowed to 18,458 results for: Met

1. pLVPT-GDNF-tTR-KRAB

   Plasmid

   #11646

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for GDNF (mPGK promoter) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GDNF, tTR-KRAB, Tet-on (GDNF Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgApc/Cre

   Plasmid

   #89641

   Purpose

   Expresses an Apc-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Apc (Apc Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. anti-Notch2_B9-pBIOCAM5

   Plasmid

   #39348

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Human, Mouse)
   

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. ALC1-c005

   Plasmid

   #173092

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MacroH2A1.2-c002

   Plasmid

   #173088

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A1 (MACROH2A1 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Q162-N372, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTagRFP-T-Cx26-7

   Plasmid

   #58007

   Purpose

   Localization: Gap Junctions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Cx26 (Gjb2 Rat)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCry2PHR-mCh-Rac1

   Plasmid

   #42954

   Purpose

   Expresses optogenetically controlled Rac1

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKG-GW1

   Plasmid

   #203163

   Purpose

   Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46594

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-17 Decoy (MIR17, Mir17 Human, Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbE1k-3

    Plasmid

    #71722

    Purpose

    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    

    Promoter

    pTrc

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-iGECI

    Plasmid

    #160423

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.U6.miR-29b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46605

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-29b Decoy (MIR29B1, Mir29b-1 Human, Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Lenti-sgCdkn2a/Cre

    Plasmid

    #89644

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgKeap1/Cre

    Plasmid

    #89645

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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