We narrowed to 5,111 results for: codon optimized

1. CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171380

   Purpose

   Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized RfxCas13d
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC2A3

   Plasmid

   #132114

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A3 (SLC2A3 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting

   Plasmid

   #92141

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC25A26

   Plasmid

   #132259

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A26 (SLC25A26 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRM055 His-TEV-Cas9 6xNLS 0C

   Plasmid

   #196246

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC18A3

   Plasmid

   #132009

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC18A3 (SLC18A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139089

   Purpose

   Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB_GST-TEV-SRC(WT)

   Plasmid

   #223742

   Purpose

   Expression of recombinant protein for purification, codon optimized for Insect cells

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Tags

   GST-TEV

   Expression

   Insect

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC10A1

   Plasmid

   #131907

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC10A1 (SLC10A1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pNMSB84

    Plasmid

    #199309

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT100

    Plasmid

    #180512

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM33_Nsp15

    Plasmid

    #156481

    Purpose

    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC2A2

    Plasmid

    #132126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221_SLC25A18

    Plasmid

    #132003

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A18 (SLC25A18 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDONR221_SLC35B1

    Plasmid

    #132099

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35B1 (SLC35B1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDONR221_SLC38A9

    Plasmid

    #132070

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A9 (SLC38A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pENTR-SLC66A2_STOP

    Plasmid

    #221419

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC66A2 (PQLC1 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEE075

    Plasmid

    #176832

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    714
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC6A14

    Plasmid

    #131865

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A14 (SLC6A14 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAB1676 REPAIR.t1

    Plasmid

    #176319

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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