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We narrowed to 10,444 results for: T7

Showing: 7881 - 7920 of 10444 results

  1. pDONR221-kcnj8

    Plasmid
    #173134
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173134-plasmid-map-sequence-id-343157

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  4. Ash1-5p

    Plasmid
    #169712
    Purpose
    Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-480…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169712-plasmid-map-sequence-id-334784

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  5. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid
    #168047
    Purpose
    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
    Depositor
    Michael Nash
    Article
    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, ddFLN4, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168047-plasmid-map-sequence-id-334769

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  6. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168476-plasmid-map-sequence-id-332734

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  7. pET11a-Z-N-DiB-RM

    Plasmid
    #168475
    Purpose
    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168475-plasmid-map-sequence-id-332732

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  14. pHIs8-4b-SaDAH::G226D

    Plasmid
    #140130
    Purpose
    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli
    Depositor
    Jing-Ke Weng
    Article
    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:
    Insert
    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    Use
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    changed Gly226 to Asp226
    Promoter
    T7
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140130-plasmid-map-sequence-id-275079

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  35. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Use
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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Showing: 7881 - 7920 of 10444 results