We narrowed to 7,181 results for: RAP

1. pBHM2404

   Plasmid

   #173441

   Purpose

   pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   mNeonGreen-CBP-2XFLAG-NAT1
   

   Use

   Tags

   CBP-2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-2xFYVE_hrs

   Plasmid

   #67676

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2xFYVEhrs
   

   Use

   Gateway multisite 3' entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK288 (mAID-Bsr)

   Plasmid

   #72826

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Bsr
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBHM2406

   Plasmid

   #173442

   Purpose

   pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   mNeonGreen-CBP-2XFLAG-HYGB
   

   Use

   Tags

   CBP-2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #219782

   Purpose

   CaST-IRES for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-16

   Plasmid

   #172746

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-noPS

   Plasmid

   #177943

   Purpose

   Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Use

   Tags

   His6-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta-actin promoter

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-JEDI-1P-WPRE

    Plasmid

    #202613

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBXNPHM3

    Plasmid

    #110099

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-HA-GASC1

    Plasmid

    #24214

    Purpose

    Mammalian expression of human JMJD2C with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    GASC1 (KDM4C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFosCreER

    Plasmid

    #46388

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Fos (Fos Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_PGK_SNIPR_hsNotch3 LNR

    Plasmid

    #188338

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_PGK_SNIPR_FAP site with scFv

    Plasmid

    #188351

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXJ-His-VTCN1

    Plasmid

    #232664

    Purpose

    transient expresses VTCN1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    VTCN1 (VTCN1 Human)
    

    Use

    Tags

    His and His (retained after signal peptide cleava…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65252

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA041

    Plasmid

    #215963

    Purpose

    Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EFS_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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