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We narrowed to 772 results for: tev cleavage

Showing: 51 - 100 of 772 results

  11. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

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  12. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid
    #29656
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, MBP, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29656-plasmid-map-sequence-id-237947

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  14. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  16. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid
    #29663
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, GFP, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29663-plasmid-map-sequence-id-197189

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  22. pmalc4x_E323K_TEV

    Plasmid
    #47699
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed glutamic acid 323 to lysine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47699-plasmid-map-sequence-id-68104

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  24. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Use
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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  31. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  32. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  38. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  39. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid
    #90474
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    90474-plasmid-map-sequence-id-181091

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  40. pET28a-hfYFP-TEV-SAV

    Plasmid
    #191197
    Purpose
    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    hfYFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191197-plasmid-map-sequence-id-376977

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  41. pET28a-LSSmGFP-TEV-SAV

    Plasmid
    #191200
    Purpose
    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191200-plasmid-map-sequence-id-376986

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  43. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119299-plasmid-map-sequence-id-231827

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  47. pET24a-VHH-tev-mCherry

    Plasmid
    #117752
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117752-plasmid-map-sequence-id-235994

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Showing: 51 - 100 of 772 results