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We narrowed to 619 results for: ubiquitin plasmid

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Showing: 51 - 100 of 619 results

  1. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available Since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170774-plasmid-map-sequence-id-339008

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  2. pUltra-puro-RTTA3

    Plasmid
    #58750
    Purpose
    silence doxycyclin inducible pUltra-dox
    Depositor
    Yildirim Dogan, Kitai Kim
    Insert
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Ubiquitin C promoter
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    58750-plasmid-map-sequence-id-92492

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  3. LvPolyubiquitin-GRX1-roGFP2-minos-lox-ITR

    Plasmid
    #247292
    Purpose
    Polyubiquitin promoter driving expression of glutathione biosensor GRX1-roGFP2. Flanked by ITRs compatible with minos-mediated transgenesis
    Depositor
    Amro Hamdoun
    Insert
    GRX1-roGFP2
    Use
    Synthetic Biology; Sea urchin expression
    Promoter
    Polyubiquitin
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247292-plasmid-map-sequence-id-491315

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  4. LvPolyubiquitin-roGFP2-ORP1-minos-lox-ITR

    Plasmid
    #247293
    Purpose
    Polyubiquitin promoter driving expression of cytoplasmic hydrogen peroxide biosensor roGFP2-ORP1. Flanked by ITRs compatible with minos-mediated transgenesis.
    Depositor
    Amro Hamdoun
    Insert
    roGFP2-ORP1
    Use
    Synthetic Biology; Sea urchin expression
    Tags
    Grx1
    Promoter
    Polyubiquitin
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247293-plasmid-map-sequence-id-491309

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  8. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  9. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available Since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

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  10. PP-UBC13 (2R0J)

    Plasmid
    #25114
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFE1350c:C34884 (PFE1350c Plasmodium falciparum)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 3-150
    Available Since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. DTX3L

    Plasmid
    #28266
    Depositor
    Cheryl Arrowsmith
    Insert
    DTX3L (DTX3L Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains only carboxyl terminal domain (residues …
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    28266-plasmid-map-sequence-id-463306

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  13. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pET-UBE3A iso 2 N terminus

    Plasmid
    #185643
    Purpose
    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells
    Depositor
    Irina Bezsonova
    Insert
    ubiquitin-protein ligase E3A (UBE3A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    none
    Available Since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    185643-plasmid-map-sequence-id-367329

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  16. PP-UBC13 (3E95)

    Plasmid
    #25131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFE1350c:C34878
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. PP-UEV1A

    Plasmid
    #25111
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFC0255c:C34858
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. USP21 (3I3T)

    Plasmid
    #25147
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp21.209.563 (USP21 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Available Since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. USP08 (3N3K)

    Plasmid
    #28115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP08 (USP8 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    Catalytic domain only.
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. NRDP1

    Plasmid
    #25620
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRDP1 (RNF41 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains USP8 interaction Domain
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. UBE2G1

    Plasmid
    #25614
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBE2G1 (UBE2G1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  25. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  26. UBC64

    Plasmid
    #25143
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc64.248.414 (UBE2Q1 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains UBC domain; residues 248-414
    Available Since
    Oct. 31, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. UBC44

    Plasmid
    #25468
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc44.001.167
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

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  31. 5C7M (UBC)

    Plasmid
    #69967
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubv.it.02:YTC006-E08:C225422 (UBC Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. UBH26

    Plasmid
    #25467
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh26.019.099 (UBQLN3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 19-99
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. PLXND1 (3H6N)

    Plasmid
    #25187
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXND1:A1553-D1656 (PLXND1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A1553-D1656
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. I766T USP7 UBL-12

    Plasmid
    #227232
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    I766T; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227232-plasmid-map-sequence-id-468099

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  35. L757P USP7 UBL-12

    Plasmid
    #227231
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L757P; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227231-plasmid-map-sequence-id-468098

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  36. R152C USP7 TRAF

    Plasmid
    #227229
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    R152C; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227229-plasmid-map-sequence-id-452330

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  37. G392A FL USP7

    Plasmid
    #227217
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227217-plasmid-map-sequence-id-452329

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  38. L469R USP7 catalytic

    Plasmid
    #227212
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227212-plasmid-map-sequence-id-452328

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  39. N418S FL USP7

    Plasmid
    #227215
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418S
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227215-plasmid-map-sequence-id-449605

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  40. H111Q USP7 TRAF

    Plasmid
    #227227
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    H111Q; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227227-plasmid-map-sequence-id-449602

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  41. G392D FL USP7

    Plasmid
    #227216
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392D
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227216-plasmid-map-sequence-id-449551

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  42. K420E FL USP7

    Plasmid
    #227223
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    K420E
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227223-plasmid-map-sequence-id-449550

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  43. L299A USP7 catalytic

    Plasmid
    #227213
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L299A; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227213-plasmid-map-sequence-id-449549

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  44. V485G USP7 catalytic

    Plasmid
    #227209
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    V485G; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227209-plasmid-map-sequence-id-449548

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  45. P273H USP7 catalytic

    Plasmid
    #227208
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    P273H; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227208-plasmid-map-sequence-id-449547

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  46. Y331H USP7 catalytic

    Plasmid
    #227205
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Y331H; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227205-plasmid-map-sequence-id-449546

    Enlarge

  47. M225I USP7 catalytic

    Plasmid
    #227206
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    M225I; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227206-plasmid-map-sequence-id-449545

    Enlarge

  48. USP7 N418S catalytic

    Plasmid
    #227202
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418Sl; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227202-plasmid-map-sequence-id-449544

    Enlarge

  49. L373F FL USP7

    Plasmid
    #227220
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L373F
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227220-plasmid-map-sequence-id-449541

    Enlarge

  50. Y331H FL USP7

    Plasmid
    #227218
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Y331H
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227218-plasmid-map-sequence-id-449540

    Enlarge

Showing: 51 - 100 of 619 results