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Plasmid#187952PurposeFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-CasRx-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linker, HA-tag, and NLSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBlueKan+cysMPro
Plasmid#187879PurposeEscherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterCysMAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorInsertNDP52 (CALCOCO2 Human)
UseTagsGST-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV IRES Luciferase
Plasmid#18760DepositorTypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPL13-ECFP-SNAP
Plasmid#187967Purposeexpresses RPL13 with C-terminal tags ECFP and SNAPDepositorInsertRPL13 (RPL13 Human)
UseTagsECFP-SNAPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TYRO3
Plasmid#187793PurposeMAC-tagged gene expression of human TYRO3DepositorInsertTYRO3 (TYRO3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast
Plasmid#187320PurposedCas9 expression vector with cloning site for C terminal fusionsDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SUN2 mApple
Plasmid#187679PurposeIn vivo visualization of SUN2DepositorInsertSUN2 (Sun2 Mouse)
UseTagsmAppleExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SS-HA-Sun1L-KDEL
Plasmid#187016PurposeDisruption of LINC ComplexDepositorInsertSS-HA-Sun1L-KDEL
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Nesprin-1αKASH
Plasmid#187015PurposeDisruption of LINC ComplexDepositorInsertNesprin-1alphaKASH
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SUN2 mScarlet
Plasmid#187685PurposeIn vivo visualization of SUN2DepositorInsertSUN2 (Sun2 Mouse)
UseTagsmScarletExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only