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Showing: 21 - 40 of 30033 results

  4. iCAP-ROOT

    Plasmid
    #192262
    Purpose
    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid
    Depositor
    Li Ye
    Article
    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:
    Insert
    Synthetic construct for ROOT VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p41
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192262-plasmid-map-sequence-id-379257

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  6. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  12. lentiCas9-Venus

    Plasmid
    #70267
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    Cas9
    Venus Reporter
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS and P2A
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70267-plasmid-map-sequence-id-126420

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  14. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Mutation
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  20. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Article
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Insert
    MiniCAFE
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    Dpy-30
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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Showing: 21 - 40 of 30033 results