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Showing: 951 - 1000 of 17679 results

  6. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  7. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid
    #92299
    Purpose
    Expression of humanized CMaCpf1
    Depositor
    Feng Zhang
    Insert
    huCMaCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92299-plasmid-map-sequence-id-181633

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  8. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139168-plasmid-map-sequence-id-309358

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  14. pSBBi-GB GST-CaXMT1

    Plasmid
    #139169
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139169-plasmid-map-sequence-id-309359

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  20. pTGD

    Plasmid
    #88894
    Purpose
    Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli
    Depositor
    Keith Tyo
    Article
    Love et al Methods Mol Biol. 2019;1927:37-45. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    88894-plasmid-map-sequence-id-174381

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  22. pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT

    Plasmid
    #139176
    Purpose
    SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.
    Depositor
    Neal Devaraj
    Insert
    CaMXMT
    Use
    Transposon
    Tags
    dTomato-P2A
    Expression
    Mammalian
    Mutation
    P131T on dTomato (Please see depositor comments)
    Promoter
    tight TRE-controlled CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139176-plasmid-map-sequence-id-309363

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Showing: 951 - 1000 of 17679 results