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Addgene

We narrowed to 1,181 results for: grna cloning vector

Showing: 951 - 1000 of 1181 results
  1. pYJA5

    Plasmid
    #217778
    Purpose
    The empty vector for quadruple sgRNA cloning
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Puromycin resistance gene, T2A, and TagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    PGK, CMV, U6
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid
    #173169
    Purpose
    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site
    Depositor
    Insert
    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, amino acids …
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid
    #173167
    Purpose
    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation
    Depositor
    Insert
    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    Use
    Retroviral
    Tags
    3XFLAG
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, G2A, S6A
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid
    #178209
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HA and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid
    #178211
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    Nuclear Localization Signal and Ollas.S.V5
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid
    #178224
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pLKO_HA.NWS.Ollas_mCherry-NLS

    Plasmid
    #178263
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.NWS.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pLKO_NWS.HA.V5_mCherry-NLS

    Plasmid
    #178283
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid
    #178236
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.VSVg.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid
    #178234
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pLKO_C.HA.V5_mCherry-NLS

    Plasmid
    #178242
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid
    #178220
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pLKO_AU1.HA.S_mCherry-NLS

    Plasmid
    #178219
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pLKO_AU1.HA.HSV_mCherry-NLS

    Plasmid
    #178216
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pLKO_AU1.C.VSVg_mCherry-NLS

    Plasmid
    #178214
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pLKO_AU1.C.S_mCherry-NLS

    Plasmid
    #178213
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pLKO_AU1.C.HSV_mCherry-NLS

    Plasmid
    #178210
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. pLKO_NWS.Ollas.S_mCherry-NLS

    Plasmid
    #178279
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pLKO_HSV.S.V5_mCherry-NLS

    Plasmid
    #178278
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.S.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pLKO_HSV.Ollas.V5_mCherry-NLS

    Plasmid
    #178277
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid
    #178273
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.NWS.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. pLKO_HA.S.VSVg_mCherry-NLS

    Plasmid
    #178269
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  25. pLKO_HA.HSV.VSVg_mCherry-NLS

    Plasmid
    #178261
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  26. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid
    #178260
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  27. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid
    #178255
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid
    #178246
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pLKO_C.HSV.S_mCherry-NLS

    Plasmid
    #178245
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid
    #178241
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. pLKO_C.HA.S_mCherry-NLS

    Plasmid
    #178240
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pLKO_C.HA.HSV_mCherry-NLS

    Plasmid
    #178237
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. pLKO_AU1.HSV.VSVg_mCherry-NLS

    Plasmid
    #178225
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pLKO_AU1.HSV.Ollas_mCherry-NLS

    Plasmid
    #178223
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  35. pLKO_AU1.HA.V5_mCherry-NLS

    Plasmid
    #178221
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. pLKO_AU1.HA.Ollas_mCherry-NLS

    Plasmid
    #178218
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  37. pLKO_AU1.C.V5_mCherry-NLS

    Plasmid
    #178215
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  38. pLKO_AU1.C.Ollas_mCherry-NLS

    Plasmid
    #178212
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. pLKO_HSV.Ollas.S_mCherry-NLS

    Plasmid
    #178276
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  40. pLKO_HA.HSV.Ollas_mCherry-NLS

    Plasmid
    #178259
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 951 - 1000 of 1181 results