We narrowed to 2,461 results for: T2A

1. pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

   Plasmid

   #154263

   Purpose

   Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRF1-GW mNeongreen-T2A-mCherry

   Plasmid

   #125698

   Purpose

   Produces equimolar expression of mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mNeongreen-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SOX2 KO (T2A-H2B-EGFP)

   Plasmid

   #167969

   Purpose

   SOX2 CDS replacement with T2A-H2B-EGFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 2 (SOX2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRF1-GW ymNeongreen-T2A-mTurquoise2

   Plasmid

   #125701

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mTurquoise
   

   Expression

   Yeast

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF11-wmiRFP2-T2A-HO1

   Plasmid

   #179485

   Purpose

   Expresses miRFP2 and HO1in worm cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   wmIRFP2-T2A-HO1
   

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   tag-168 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIHEU_miniSOG2 T2A H3.3A-EGFP

   Plasmid

   #87411

   Purpose

   UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

   Insert

   mini singlet oxygen generator 2
   

   Expression

   Insect

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-hCas9-T2A-miRFP670

   Plasmid

   #187223

   Purpose

   Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRF1-GW mScarletI-T2A-mTurquoise2

   Plasmid

   #118430

   Purpose

   Produces equimolar expression of mScarletI and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mScarletI-T2A-mTurquoise2
   

   Expression

   Yeast

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAav-TP53-T2A-BirA*

    Plasmid

    #115652

    Purpose

    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

    Inserts

    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    

    Use

    AAV

    Tags

    BirA* and T2A

    Mutation

    Homology region 1 and Homology region 2

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105982

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    FAPdL5-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Promoter

    human synapsin 1

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW Electra1-T2A-mCherry

    Plasmid

    #205498

    Purpose

    Expression of Electra1-T2A-mCherry in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    Electra1-T2A-mCherry
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDRF1-GW eGFP-T2A-mCherry

    Plasmid

    #118422

    Purpose

    Produces equimolar expression of eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    eGFP-T2A-mCherry
    

    Expression

    Yeast

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mScarlet-I-T2A-LSS-SGFP2

    Plasmid

    #112946

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Insert

    mScarlet-I and LSS-SGFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-pac-T2A-iCre

    Plasmid

    #113849

    Purpose

    Puromycin resistance gene and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRF1-GW mKate2-T2A-mTurquoise2

    Plasmid

    #118426

    Purpose

    Produces equimolar expression of mKate2 and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mKate2-T2A-mTq2
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL iRFP-T2A-Trex2

    Plasmid

    #50418

    Purpose

    Coexpresses Trex2 and iRFP in mammalian cells.

    Depositor

    Andrew Scharenberg

    Inserts

    iRFP
    Trex2
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. H2B-sfGFP-T2A-iCre

    Plasmid

    #178546

    Purpose

    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox and Mouse Targeting; Flp/frt

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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