We narrowed to 59,251 results for: cloning

1. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

   Plasmid

   #224500

   Purpose

   Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

   Plasmid

   #224496

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1 EGFP

   Plasmid

   #129442

   Purpose

   expression of intein/ gfp

   Depositor

   Oskar Laur

   Insert

   intein/ gfp
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSP1

   Plasmid

   #199384

   Purpose

   Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4

   Depositor

   Hang Lu

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Cloning vector backbone

   Available Since

   April 20, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMulti_S_ccdB

   Plasmid

   #223853

   Purpose

   GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Type

   Empty backbone

   Use

   Golden-gate acceptor

   Expression

   Bacterial

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT413

   Plasmid

   #8606

   Depositor

   Robert Hartley

   Article

   Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

   Insert

   barnase, barstar
   

   Tags

   phoA signal sequence to barnase

   Expression

   Bacterial

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pMT416

   Plasmid

   #8607

   Depositor

   Robert Hartley

   Article

   Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

   Insert

   barnase, barstar
   

   Tags

   phoA signal sequence to barnase

   Expression

   Bacterial

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF

    Plasmid

    #42519

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Expression

    Bacterial

    Promoter

    T7, T3

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. IMBB2.4-pUC57-mini

    Plasmid

    #88845

    Purpose

    plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR L1-20XUAS-L4

    Plasmid

    #32307

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    20XUAS
    

    Use

    Gateway multisite entry clone

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR L1-5XQUAS-L4

    Plasmid

    #32308

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    5XQUAS
    

    Use

    Gateway multisite entry clone

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR R4-QF-R3

    Plasmid

    #32310

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    QF
    

    Use

    Gateway multisite entry clone

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR L5-QF-L2

    Plasmid

    #32305

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    QF
    

    Use

    Gateway multisite entry clone

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMulti_K_ccdB

    Plasmid

    #223852

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMulti_A_ccdB

    Plasmid

    #223851

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-MCS UBC promoter

    Plasmid

    #128045

    Purpose

    Lentiviral vector with UBC promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-MCS PGK promoter

    Plasmid

    #128044

    Purpose

    Lentiviral vector with PGK promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3basic luciferase and renilla_polyA

    Plasmid

    #128046

    Purpose

    pGL3 basic luciferase reporter with co-expression of renilla luciferase

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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