Addgene

We narrowed to 2,833 results for: eed

Showing: 381 - 400 of 2833 results

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pSG84-pLas-TetRssrA

Plasmid

#102455

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pLas

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroSuper shSmad4J hygro

Plasmid

#19151

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against SMAD family member 4 (SMAD4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only
Sc eRF1 (WT)

Plasmid

#175413

Purpose

Encodes His-MBP-TEV-WT eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin B2

Plasmid

#10911

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin B2 (CCNB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pLas-tetO

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1

Plasmid

#8962

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pBSII-SK-mTol2-MCS

Plasmid

#51817

Purpose

To generate transgenic axolotls using Tol2 or SceI mediated transgenics

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSG13-pBAD-TetRssrA

Plasmid

#102449

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Kan

Plasmid

#188918

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Kan

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMTG07-Amp-FRT

Plasmid

#128276

Purpose

Gene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

Gal4-VVD-p65

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1258

Plasmid

#116898

Purpose

TetOFF:GFP:CenpA (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

CenpA promoter region
CenpA

Use

Fungal expression (in magnaporthe oryzae)

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-Syt::3xHalo7

Plasmid

#87649

Purpose

For creating drosophila transgenics expressing UAS-Syt::3xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::3xHalo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1

Plasmid

#37480

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1 second generation cameleon (calcium sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pN_35S/mApple

Plasmid

#132566

Purpose

mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mApple

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAP1698-4

Plasmid

#105242

Purpose

mammalian eGFP

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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