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mNeonGreen-JIP4

Plasmid

#164621

Purpose

Expresses JIP4 in mammalian cells with a mNeonGreen tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98928

Purpose

Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has service

AAV1 and AAV5

Insert

Ruby2-smFP-Flag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

18 Articles

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

FRB (MTOR Human)

Expression

Mammalian

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

32 Articles

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 Y701F (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Publication

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Publication

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727A (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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