We narrowed to 59,655 results for: plasmid
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AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianPromoterCbhAvailable sinceMay 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
Plasmid#51087PurposeForebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterCaMKIIa 1.3 kbAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCLIP-tag (m) Vector
Plasmid#101136PurposeExpression of protein of interest with CLIP-Tag in Mammalian cellsDepositorTypeEmpty backboneTagsCLIP-tag (CLIP10)ExpressionMammalianAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
eIF2a 2
Plasmid#21808DepositorAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAlbumin
Plasmid#22037DepositorInsertpartial sequence of human albumine
UseLentiviralAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-hChR2(H134R)-mCherry
Plasmid#27055PurposeAAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitationDepositorInserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterGFAPAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST51-LRRK2-G2019S
Plasmid#29401DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLRRK2 (LRRK2 Human)
UseGateway CloningTags6XHis and V5ExpressionMammalianMutationGly2019SerAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorPublicationTypeEmpty backboneUseRetroviralExpressionMammalianAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFYF1320 EGFP Site#1
Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA-EGFP site 1
UseCRISPRExpressionMammalianPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVExpressionMammalianPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV2/MG1.2
Plasmid#184541PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsidDepositorInsertAAV-MG1.2 capsid
UseAAV; Helper vector for packagingMutationchanges on AAV9 capsid: K449R, Q588T; insertion o…Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKu70 (XRCC6 Human)
TagsGFP-FLAGExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-TLR2-CFP
Plasmid#13015DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertToll-Like Receptor 2 (TLR2 Human)
TagsCFPExpressionMammalianAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by
