19,635 results

1. pTK168_mCherry-H2B

   Plasmid

   #46363

   Purpose

   visualizes chromosomes

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   H2B (H2BC11 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D26G, V119I and S125D

   Promoter

   pBABE

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3XMEF2-luc

   Plasmid

   #32967

   Purpose

   Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

   Depositor

   Ron Prywes

   Insert

   MEF2 binding sites (MEF2A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   MEF2

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM496 (deletions) Zeo

   Plasmid

   #109301

   Purpose

   ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   Bxb1 attB site
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWSK29

   Plasmid

   #172972

   Purpose

   Low copy number cloning vector

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOSCAR

   Plasmid

   #29639

   Depositor

   David_L Andrews, Scott Gold

   Article

   Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

   Type

   Empty backbone

   Available Since

   Sept. 19, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. En1

   Plasmid

   #34993

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Homeobox protein engrailed-1 (En1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mito cpVenus control

    Plasmid

    #186792

    Purpose

    Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PTEN

    Plasmid

    #28298

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-TRAIL

    Plasmid

    #10953

    Depositor

    Bingliang Fang

    Article

    Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

    Insert

    Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Expression

    Bacterial

    Available Since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12V mutant

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only