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pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Tags

cSAT2.0

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pNIC-CTHF

Plasmid

#26105

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

TEV - His6 - Flag

Expression

Bacterial

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pAB213

Plasmid

#101677

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-Halo-tDeg

Plasmid

#191370

Purpose

Overexpression of Halo-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

Halo-tDeg

Tags

Halo

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin2

Plasmid

#239018

Purpose

Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2-WPRE

Plasmid

#104110

Purpose

AAV-mediated expression of mTurquoise2 under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

Plasmid

#244218

Purpose

Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

Plasmid

#244204

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

Plasmid

#244219

Purpose

Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV-LCMV-WE

Plasmid

#15793

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pPSU100

Plasmid

#173835

Purpose

Expresses 100 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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lentiGuide-tagBFP

Plasmid

#217007

Purpose

lentiviral plasmid for gRNA cloning with selectable tagBFP expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKanCMV_mRuby3

Plasmid

#172854

Purpose

mRuby3 expressed under the CMV promotor

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

mRuby3

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pGL1_39

Plasmid

#199046

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL0_1 [RK2 ORI]

Plasmid

#198918

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI

Use

Synthetic Biology

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Cortactin-pmCherryC1

Plasmid

#27676

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cortactin (Cttn Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pGL0_5 [pAL5000 ORI]

Plasmid

#198922

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EYFP-WPRE

Plasmid

#104111

Purpose

AAV-mediated expression of EYFP under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pREXC3GH

Plasmid

#47077

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site, GFP, and His

Promoter

NIS (nisin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Tags

T7 tag and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVlux-cp157Venus

Plasmid

#197584

Purpose

BRET lux luciferase with cp157Venus for mammalian expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

human codon-optimized Lux-cp157Venus

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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73-21 KRAS PANCS-binder posAP

Plasmid

#240119

Purpose

KRAS G12D PANCS-binder positive AP

Depositor

Bryan Dickinson

Article

Styles et al bioRxiv [Preprint]. 2025 Jan 17:2025.01.

Insert

KRas-RNAPC Pos AP fusion

Expression

Bacterial

Promoter

custom

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-hCALML3

Plasmid

#47605

Depositor

Emanuel Strehler

Article

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CALML3 (CALML3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pENTR1a-CRISPRoffv2.1

Plasmid

#207174

Purpose

Gateway Cloning Vector for CRISPRoffv2.1

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

CRISPRoffv2.1

Use

CRISPR

Tags

TagBFP

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNC-Cam2304-RNAi

Plasmid

#193400

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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