We narrowed to 14,660 results for: flag
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Plasmid#213194PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pINTO-NFH::mEzh2
Plasmid#65925PurposeExpression of mouse Ezh2 fused to Flag-HA tag (N-terminus)DepositorInsertEnhancer of zeste homolog 2 (Ezh2 Mouse)
TagsFLAG-HAExpressionMammalianPromoterCMV/TO (T-REx) and CMV/TetO2 (T-REx)Available SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp12
Plasmid#157710Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nprot
Plasmid#157729Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp9
Plasmid#157704Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp10
Plasmid#157707Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp9-HF
Plasmid#157705Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HTR1D-DuET
Plasmid#213318PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13-HF_GC3opt
Plasmid#157716Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
TagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HTR6-DuET
Plasmid#213326PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp5-HF
Plasmid#157687Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST40-2XFL-Src-S4-S32-KD
Plasmid#140299PurposeExpresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationlacks the UD regionPromoterCMVAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp14-HF
Plasmid#157719Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
BDKBR1-DuET
Plasmid#213189PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13_GC3opt
Plasmid#157715Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
TagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpCas9-NRCH-APOBEC-nCas9-Ung
Plasmid#198552PurposeExpresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13
Plasmid#157713Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF
Plasmid#201217PurposepCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTFDepositorAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp12-HF
Plasmid#157711Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FPR3-DuET
Plasmid#213241PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp5
Plasmid#157686Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Orf6
Plasmid#157727Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp2
Plasmid#157683Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCALNL-mutKir2.1
Plasmid#125579PurposeCre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationA non-conducting mutant (GYG -> AAA).Available SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPR20-DuET
Plasmid#213275PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MTOR HEAT
Plasmid#120709PurposeExpresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus productionDepositorInsertMTOR HEAT 1-9 (MTOR Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 1-409PromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPR1-DuET
Plasmid#213257PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GABPA
Plasmid#64253PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPADepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTR2C-DuET
Plasmid#213323PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HTR4-DuET
Plasmid#213324PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CALCRb-DuET
Plasmid#213193PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-HF
Plasmid#157696Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBacMam2-DiEx-LIC-C entry vector
Plasmid#111752PurposeIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cellsDepositorTypeEmpty backboneUseBaculovirus expressionTagsFLAGExpressionMammalianAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
ADRA2A-DuET
Plasmid#213177PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD5-DuET
Plasmid#213231PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM4-DuET
Plasmid#213210PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only