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pVRC8400-SARS-CoV-2-RBD-SD1-AVI

Plasmid

#160477

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-SD1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV Sport2 mPer2

Plasmid

#16204

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per2 (Per2 Mouse)

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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HA-TEAD1

Plasmid

#229411

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

TEAD1 (TEAD1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDZ138

Plasmid

#41949

Depositor

Stephen Elledge

Article

Zhang et al Cell. 2006 Aug 11;126(3):529-42.

Insert

ubiquitin specific peptidase 28 (USP28 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C171A

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, William Hahn, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP

Plasmid

#108418

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

PQ5 (cumate-inducible)

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L

Plasmid

#27000

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE60

Plasmid

#247036

Purpose

Lentiviral expression of a minimal promoter only with single TATA cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-EGFP

Plasmid

#177136

Purpose

Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

EGFP

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-LEXY (pDN122)

Plasmid

#72655

Purpose

Blue light-inducible nuclear export of mCherry

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-LEXY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-hADAR2dd-pAG

Plasmid

#248103

Purpose

Expresses hADAR2dd fused to N-terminus of Protein A/G in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

hADAR2dd (ADARB1 Human)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-pAG

Plasmid

#248102

Purpose

Expresses rAPOBEC1 fused to N-terminus of Protein A/G in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-hADAR2dd-pAG

Plasmid

#248101

Purpose

Expresses rAPOBEC1-hADAR2dd-pAG in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
hADAR2dd (ADARB1 Human)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2922 [pNP2321 - Cloning backbone, bRNA Landing Pad, with Ef1a-ISCro4 Recombinase]

Plasmid

#247255

Purpose

Cloning backbone for cloning bridge RNAs for ISCro4

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pATUBR

Plasmid

#245488

Purpose

Binary Plasmid for plant transformation expressing RUBY from the Arabidopsis thaliana polyubiquitin 10 gene promoter and the terminator from the pea RBCS gene. Kanamycin plant selection

Depositor

Beth Rowan

Insert

pAtUBI-RUBY-tRBCS

Expression

Plant

Promoter

Polyubiquitin 10 gene from Arabidopsis thaliana

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMef2c-AHF-DEST (JDW 476)

Plasmid

#242577

Purpose

Gateway destination vector with the murine Mef2c anterior heart field promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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HiBiT-KEAP1(CMV) Vector

Plasmid

#238612

Purpose

Express HiBiT-KEAP1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

KEAP1 (KEAP1 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV_N7-BE4-puro

Plasmid

#226584

Purpose

Express N7-BE4 base editor in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

N7-BE4

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-E2-ABE-Cas9n-puro

Plasmid

#226587

Purpose

Express E2-ABE in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

E2-ABE

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-PECAM1-Neo

Plasmid

#201054

Purpose

Endothelial promoter driven neomycin selection cassette

Depositor

Paul Burridge

Article

Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

Insert

Adeno-associated virus integration site 1 (AAVS1 Human)

Use

AAV

Tags

Neomycin resistance

Promoter

PECAM1

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXO31

Plasmid

#236429

Purpose

transient overexpression of FBXO31 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

FBXO31 (FBXO31 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher003:mNG

Plasmid

#225190

Purpose

Used for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher003:mNeonGreen

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT47

Plasmid

#223419

Purpose

T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_FAM153CP_SOX9rep

Plasmid

#232400

Purpose

Enhancer near FAM153CP, buffering element replaced with SOX9 site

Depositor

Joanna Wysocka

Insert

FAM153CP (FAM153CP Human)

Use

Luciferase

Mutation

Enhancer near FAM153CP, buffering element replace…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcRII-mogat3a in situ probe

Plasmid

#232135

Purpose

zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3a

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHSP90(G97D)-mOFP

Plasmid

#228195

Purpose

expressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP90AA1(G97D) (HSP90AA1 Human)

Tags

mOFP

Expression

Mammalian

Mutation

G97D mutation

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7368

Plasmid

#217375

Purpose

sfGFP reporter for Bce crcB fluoride riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

Bce crcB riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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