We narrowed to 30,468 results for: Ide

1. pLyGo-Ec-3

   Plasmid

   #163129

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tol2-GgVCL/*TBM

   Plasmid

   #162790

   Purpose

   MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
   

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   changed Gly 454 to Val

   Promoter

   beta actin

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLyGo-Ec-5

   Plasmid

   #163131

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDUAL_2_lacZ

   Plasmid

   #155173

   Purpose

   see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDUAL_araBAD

   Plasmid

   #155172

   Purpose

   BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDUAL_2_tacI

   Plasmid

   #155174

   Purpose

   see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDUAL_lacZ

   Plasmid

   #155169

   Purpose

   BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBICI_2_medium

   Plasmid

   #155167

   Purpose

   see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBICI_strong

   Plasmid

   #155165

   Purpose

   BaSP and CuCBP co-expression: strong RBS controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBICI_medium

    Plasmid

    #155164

    Purpose

    BaSP and CuCBP co-expression: medium RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBICI_weak

    Plasmid

    #155163

    Purpose

    BaSP and CuCBP co-expression: weak RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. D70_Ld_210610

    Plasmid

    #135883

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    dihydrolipoamide acetyltransferase precursorlike protein
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. D6_Ld_100150

    Plasmid

    #135822

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    nucleoside phosphatase, putative
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX307-VHL(C162F)-3F-BirA

    Plasmid

    #220149

    Purpose

    Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed cysteine 162 to phenylalanine; codon opti…

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

    Plasmid

    #206461

    Purpose

    Oskar 139-240 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 139-240 (osk Fly)
    

    Tags

    PH mcherry

    Expression

    Insect

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC3.1_CMV_oROS-G

    Plasmid

    #216111

    Purpose

    Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC3.1_CAG_oROS-Gr_LF(C199S)

    Plasmid

    #216114

    Purpose

    Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-Gr_LF(C199S)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge