We narrowed to 13,509 results for: GAN

1. pRSF-G1(6CNW)RS

   Plasmid

   #222873

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCS.71/2272.Hygro

   Plasmid

   #22731

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

   Insert

   Lox71-FRT-PGK-hygro-FRT-Lox2272
   

   Use

   Cre/Lox

   Mutation

   This is a basal exchange vector for RMCE. The vec…

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-mRuby-Rab8a

   Plasmid

   #194337

   Purpose

   Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Rab8 (RAB8A Canis lupus)
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-hArfaptin1

   Plasmid

   #206404

   Purpose

   Mammalian expression of hArfaptin1

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

   Insert

   Arfaptin1 (ARFIP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B2301

   Plasmid

   #91067

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   35S

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBacDual with LFn + Needle

   Plasmid

   #84868

   Purpose

   Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Needle
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   AtU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-AR-174-566

   Plasmid

   #104197

   Purpose

   Expresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GST

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   human androgen receptor aa 174-566 (AR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEDF-PhdRS

    Plasmid

    #127445

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Expression

    Bacterial

    Mutation

    N346A-C348A mutations on PylRS

    Promoter

    araBAD

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmPB2-VSV

    Plasmid

    #68038

    Purpose

    Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B2 (Plxnb2 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. STARR-seq luciferase validation vector_SCP1_empty

    Plasmid

    #99299

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMP11-bio-His

    Plasmid

    #53408

    Purpose

    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMP11 (MMP11 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.VTN.C-HA

    Plasmid

    #154849

    Purpose

    Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Vitronectin (Vtn Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TetO-FUW-PLAGL1

    Plasmid

    #61542

    Purpose

    Expresses PLAGL1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    PLAGL1 (PLAGL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFastBacDual with LFn + Fla

    Plasmid

    #84866

    Purpose

    Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Use

    Baculovirus

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_GNA15_G-alpha

    Plasmid

    #109782

    Purpose

    Protein expression and purification of GNA15_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA15_G-alpha (GNA15 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMOD_B2615

    Plasmid

    #91078

    Purpose

    Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

25. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pMCS.71/2272

    Plasmid

    #22732

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-MCS-Lox2272
    

    Use

    Cre/Lox

    Mutation

    This is a basal exchange vector for RMCE containi…

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. pB-RTP2-Integration

    Plasmid

    #129459

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP2 (RTP2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE (Tet-On)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. SERPINA-bio-His

    Plasmid

    #52182

    Purpose

    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINA (SERPINA1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CBP TAZ1(340-439)

    Plasmid

    #173760

    Purpose

    bacterial expression of mouse CBP TAZ1 domain

    Depositor

    Peter Wright

    Article

    De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

    Insert

    CBP TAZ1 (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. HA-VHL Y98H-pRc/CMV

    Plasmid

    #22044

    Depositor

    William Kaelin

    Article

    Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    point mutant, converting aa 98 from Tyrosine to H…

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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31. pET28a(+) 6xHis-TEV-IFIT3

    Plasmid

    #53559

    Purpose

    bacterial expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pHH0103_HNF4G_Hormone-recep

    Plasmid

    #109916

    Purpose

    Protein expression and purification of HNF4G_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    HNF4G_Hormone-recep (HNF4G Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103_ALDOA_Glycolytic

    Plasmid

    #109854

    Purpose

    Protein expression and purification of ALDOA_Glycolytic

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ALDOA_Glycolytic (ALDOA Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TetO-FUW-Hes5

    Plasmid

    #61536

    Purpose

    Expresses Hes5 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Hes5 (Hes5 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Expression

    Mammalian

    Promoter

    CMV-F, T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MMRN1-bio-His

    Plasmid

    #53409

    Purpose

    Expresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMRN1 (MMRN1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168170

    Purpose

    Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
    

    Tags

    YFP

    Expression

    Worm

    Mutation

    E497K and C566D

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_PCNA_PCNA

    Plasmid

    #110022

    Purpose

    Protein expression and purification of PCNA_PCNA

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PCNA_PCNA (PCNA Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMTTH+H1.4ΔCTD

    Plasmid

    #157797

    Purpose

    Expression of human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4ΔCTD (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

    Plasmid

    #32483

    Purpose

    Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    ChR2HA-2a-PSAML141F,Y115F:GlyR
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    ChR2 2A

    Promoter

    CAG

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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