We narrowed to 29,711 results for: PLE
-
Plasmid#194900PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.6
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No7
Plasmid#194901PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.7
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pA-RFP-rG2
Plasmid#188969PurposeIPTG inducible mCherry with sgRNADepositorInsertsmCherry
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyExpressionBacterialPromoterPtrcAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2
Plasmid#188964PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_ReBphP-PCM_IRES_sfGFP
Plasmid#166917PurposeExpress ReBphP-PCM and sfGFPDepositorInsertsReBphP-PCM
sfGFP
ExpressionMammalianPromoterCMVAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
ExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
ExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS62(RS)
Plasmid#123153PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS62, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS67(RS)
Plasmid#123154PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109. ParsR-ABS67, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 314
Plasmid#98584PurposepCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).DepositorInsertsCsrA
csrB
ExpressionBacterialPromoterpLacOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode81
Plasmid#83758Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode81
UseRetroviralMutationdeletion of T in starting ATG of GFP (please see …Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode71
Plasmid#83748Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode71
UseRetroviralMutationdeletion of AA5-233 in GFP (please see depositor …Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE NLS
Plasmid#82465PurposeExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminusDepositorInsertCapping Enzyme (Rngtt Mouse)
TagsMycExpressionMammalianMutationdeleted amino acids Nuclear Localization SignalPromoterCMVAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE
Plasmid#82463PurposeExpression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cellsDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only