We narrowed to 5,566 results for: SPI

1. pIW095

   Plasmid

   #134131

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1 ; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   R155A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIW090

   Plasmid

   #134129

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1 ; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   K223A K226A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIW093

   Plasmid

   #134137

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, R155A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIW082

   Plasmid

   #134136

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, R236A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIW088

   Plasmid

   #134135

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, K223A K226A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIW085

   Plasmid

   #134134

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, K183A K184A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Thy1.1-muSOX D219A

   Plasmid

   #131704

   Purpose

   encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-muSOX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Aspartic acid 219 mutated to an alanine

   Promoter

   CMV

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC0.007

   Plasmid

   #119671

   Purpose

   Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   Pcpc560-Dx2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   bp 89 T to C, bp 187 C to A, Duplicated downstrea…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pC0.006

   Plasmid

   #119670

   Purpose

   Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   Pcpc560-Ux2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   bp 89 T to C, bp 187 C to A, Duplicated upstream …

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-puro-eGFP-HEC1-d80

    Plasmid

    #114025

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-hygro-eGFP-HEC1-d80

    Plasmid

    #114030

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-hygro-eGFP-HEC1-8A/S15D

    Plasmid

    #114031

    Purpose

    Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 8A/S15D

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry_C1 I3

    Plasmid

    #61398

    Purpose

    Expresses mCherry-tagged Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5FRT/TO-GFP-I3

    Plasmid

    #61401

    Purpose

    Expresses GFP-tagged Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pT7 cyclin A1-164 venus cks1 (2196)

    Plasmid

    #61839

    Purpose

    expression of N terminal cyclin A and cks1

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    L2V

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMSCV-puro-mMCL1 Delta C22

    Plasmid

    #45820

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    mMCL-1 Delta C22
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal 22 amino acids are deleted from full l…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pMSCV-puro-Delta N67-mMCL1

    Plasmid

    #45819

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    Delta N67-mMCL-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-terminus 67 amino acids deleted from full lengt…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pBABE-miR-28

    Plasmid

    #35686

    Depositor

    George Calin

    Article

    Almeida et al Gastroenterology. 2012 Jan 10.

    Insert

    hsa-miR-28 (MIR28 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. SDHC-pHLuorin-sems

    Plasmid

    #107767

    Purpose

    labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit C of SDH fused to pHluorin (SDHC Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L2092-GPA-FullBRET-Retrovirus-TD142

    Plasmid

    #222877

    Purpose

    Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-NanoLuc-CyOFP1
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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