Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,925 results for: crispr

Clear keywords
Showing: 4021 - 4040 of 24925 results

  4. pCMV-dCas9-D3A

    Plasmid
    #78256
    Purpose
    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78256-plasmid-map-sequence-id-150190

    Enlarge

  5. pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa guide guide (pRCA594 backbone) 666

    Plasmid
    #238186
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Publication
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    mTagBFP2
    Available since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238186-plasmid-map-sequence-id-476349

    Enlarge

  6. pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

    Plasmid
    #238187
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Publication
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    mTagBFP2
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238187-plasmid-map-sequence-id-474450

    Enlarge

  9. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70018-plasmid-map-sequence-id-124557

    Enlarge

  12. pCMV/3' Box_(GLuc)_TOP1

    Plasmid
    #68434
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68434-plasmid-map-sequence-id-120212

    Enlarge

  14. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

    Enlarge

  15. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid
    #102898
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA-5guides-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102898-plasmid-map-sequence-id-198364

    Enlarge

  19. TetO-dCas9-D3A

    Plasmid
    #78254
    Purpose
    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    78254-plasmid-map-sequence-id-182128

    Enlarge

Showing: 4021 - 4040 of 24925 results