We narrowed to 7,648 results for: RAP

Showing: 4041 - 4060 of 7648 results

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pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-SIINFEKL

Plasmid

#174601

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, SIINFEKL (ovalbumin epitope)

Expression

Mammalian

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pphiLOV3-N1

Plasmid

#178973

Purpose

Expresses phiLOV3 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

phiLOV3

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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YE2-BE4max

Plasmid

#138156

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE2)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMMLV-mRFP1-P2A-Puro

Plasmid

#160228

Purpose

Expresses mRFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

mRFP1-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Multisite gateway entry clone

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

Plasmid

#199360

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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AA012

Plasmid

#215974

Purpose

Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN PLEKHM1-HA

Plasmid

#89300

Purpose

For expression of PLEKHM1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 4

Plasmid

#188364

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR tdTomato L5-L2

Plasmid

#62185

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2, G318A

Plasmid

#188365

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-cyOFP-WPRE

Plasmid

#240050

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein cyOFP from the CAG promoter

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

cyOFP-H

Use

AAV

Promoter

CAG

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMMLV-EBFP-P2A-Puro

Plasmid

#160227

Purpose

Expresses EBFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EBFP-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

Plasmid

#188377

Purpose

Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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