164,577 results
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Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Puro.Cre empty vector
Plasmid#17408PurposeLentiviral expression of Cre from the mouse PGK promoterDepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromotermouse PGKAvailable SinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163055PurposeGrx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SFNp-mRFP1
Plasmid#238473PurposeExpresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backboneDepositorInsertSFNp-mRFP1
UseTagsExpressionMutationPromoterSFNpAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYN4+
Plasmid#78715Purposemammalian expression of NHE1DepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl1
Plasmid#86678PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b
Plasmid#215820PurposeExpress CBE6b (with SpCas9) in mammalian cellsDepositorAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC16A3
Plasmid#131899PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorAvailable SinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM1-B-GRAM-H-NeoR
Plasmid#211709PurposeLentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)DepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis
Plasmid#232344PurposeExpresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tagDepositorInsertHDGF Like 2 (HDGFL2 Human)
UseTagsGGGS linker, 6xHis and Twin-strep, TEV protease s…ExpressionMammalianMutationTDP-43 regulated cryptic exonPromoterAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis
Plasmid#232343PurposeExpresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tagDepositorInsertHDGF like 2 (HDGFL2 Human)
UseTagsGGGS linker, 6xHis and Twin-strep, TEV protease s…ExpressionMammalianMutationPromoterAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-VP64dCas9VP64-2A-MPH
Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRTagsExpressionMutationPromoterAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AsdCas12a KRAB
Plasmid#188502PurposeExpresses FLAG tagged AsdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD908APromoterEF1aAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP
Plasmid#11680DepositorAvailable SinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP
Plasmid#172221PurposeCas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.DepositorTypeEmpty backboneUseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only