We narrowed to 11,735 results for: pcDNA

1. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

   Plasmid

   #231907

   Purpose

   For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-P2A-dTomato-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Const.4 EGFP-IRES-Luc-PS9

   Plasmid

   #231908

   Purpose

   For packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-IRES-Luc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Vinculin-Clover-CC-Mad-mRuby2

   Plasmid

   #233239

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-Mad-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Vinculin-Clover-CC-S0-mRuby2

   Plasmid

   #233233

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S0-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mproSAAS (21 kDa)

   Plasmid

   #231634

   Purpose

   Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

   Depositor

   Iris Lindberg

   Article

   Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

   Insert

   Pcsk1N (Pcsk1n Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of C-terminus (aa181-225)

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SARS-CoV spike all5KO mutant

   Plasmid

   #231921

   Purpose

   For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike all5KO (S SARS-CoV)
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   N1080Q, N1116Q, N1140Q, N1155Q, N1176Q

   Promoter

   CMV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MERS-CoV spike all7KO mutant

   Plasmid

   #231924

   Purpose

   For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike all7KO (S MERS-CoV)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…

   Promoter

   CMV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 Synthetic, S. pyogenes)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pt NaV1.4

   Plasmid

   #200233

   Purpose

   Expression in mammalian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

   Insert

   Phyllobates terriblis NaV1.4 – muscle channel
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dt NaV1.4

    Plasmid

    #200250

    Purpose

    Expression in mammalian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

    Insert

    Dendrobates tinctorius NaV1.4 – muscle channel
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L2383-FRB-GPA-NLuc

    Plasmid

    #222902

    Purpose

    Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cas9-FCPF

    Plasmid

    #220141

    Purpose

    Expresses Cas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

    Insert

    Cas9-FCPF (cas9 S. Pyogenes, Synthetic)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 2x3APWWP-IFP2.0C

    Plasmid

    #215752

    Purpose

    Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2x3APWWP-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2xCBX7CD-IFP2.0C

    Plasmid

    #215754

    Purpose

    Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xCBX7CD-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NSP3C-FR-2Strep

    Plasmid

    #210312

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SARS-CoV-2 N-2Strep

    Plasmid

    #210376

    Purpose

    Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-COV-2 N (N SARS-CoV-2)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TLNRD1_2T_eGFP

    Plasmid

    #208883

    Purpose

    Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations L191T and A225T

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TLNRD1_2E_eGFP

    Plasmid

    #208884

    Purpose

    Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations K192E and R233E

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. REEP6-2Strep

    Plasmid

    #210351

    Purpose

    Expresses REEP6 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP6 (REEP6 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHDR-PMG (pXK-1650)

    Plasmid

    #208858

    Purpose

    HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MCP-mCherry-TIS11B

    Plasmid

    #207670

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    TIS11B (ZFP36L1 Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFH2.17_GagZip-MCP-dpol-P2A-eGFP

    Plasmid

    #205532

    Purpose

    Express HIV GagZip-MCP-dpol-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP-dpol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper, pol fr…

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eGFP-RAPH1-F4

    Plasmid

    #194863

    Purpose

    Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1062-1250

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. eGFP-RAPH1-F3

    Plasmid

    #194862

    Purpose

    Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 868-1062

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eGFP-RAPH1-F2

    Plasmid

    #194861

    Purpose

    Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 535-868

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pClathrin-miRFP718nano

    Plasmid

    #195747

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-clathrin light chain
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTubulin-miRFP718nano

    Plasmid

    #195746

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human alpha-tubulin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FLAG-mVenusC-14-HP1b Chromo W42A

    Plasmid

    #191399

    Purpose

    W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo W42A
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    W42A

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. nPPARg429-gs10-NLS-VPR

    Plasmid

    #188266

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nPPARg429 (PPARG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin-2

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid

    #169530

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNIR-Fb21

    Plasmid

    #184679

    Purpose

    NIR-Fb to spike-Cov-19 RBD

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb21 (S Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVH63-Tier1-PMLP-Citrine_p2a_SEAP

    Plasmid

    #169557

    Purpose

    Tier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PMLP-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PMLP

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVH65-Tier1-PTK-Citrine_p2a_SEAP

    Plasmid

    #169559

    Purpose

    Tier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PTK-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PTK

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid

    #169529

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTS2388-Tier1-PhCMV-p2a_Ypet

    Plasmid

    #169525

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused yellow fluorescent protein Ypet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid

    #169524

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTS2389-Tier1-PhCMV-p2a_mRuby

    Plasmid

    #169526

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTS2406-Tier1-PhCMV-NTEV

    Plasmid

    #169501

    Purpose

    Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven N-terminal fragment of split tobacco etch virus protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

    Plasmid

    #169499

    Purpose

    Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRBSA

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

    Plasmid

    #169498

    Purpose

    Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. BirA-Myc_N_FOXL1

    Plasmid

    #167703

    Purpose

    Expresses FOXL1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FOXL1 (FOXL1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOME0135

    Plasmid

    #178397

    Purpose

    KSHV Kaposincluster-RevCompl-Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Kaposincluster-RevCompl-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pOME0134

    Plasmid

    #178396

    Purpose

    Expresses KSHV Upstream Mir-SYN

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV Upstream Mir SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge