We narrowed to 14,039 results for: TIM

1. pQZ210

   Plasmid

   #106414

   Purpose

   Co-expresses trans SNARE complex with pDC183 in E.coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Inserts

   Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
   Syntaxin-1A fragment 191-256 (Stx1a Synthetic, Rat)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Fragment 191-256 , Codon optimization and Fragmen…

   Promoter

   T7

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc Separase C2029A

   Plasmid

   #59822

   Purpose

   Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(DAP10)

   Plasmid

   #177904

   Purpose

   Bacterial expression of his10-tagged human DAP10 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   DAP10 (aa 70-92 only) (HCST Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19.TRIM28.FKBP.knock-in.mCerulean

   Plasmid

   #185009

   Purpose

   For knocking in FKBP F36V P2A mCerulean

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mCerulean
   

   Use

   Cloning

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBI-CMV1-mir451~144-CMV2-mir1

   Plasmid

   #164666

   Purpose

   Express miR-451~144 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-451~144 (MIR451A Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAav-MCS-PQS2-3xHA

   Plasmid

   #84917

   Purpose

   rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Insert

   LOX-PGK-NEO-LOX
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Promoter

   mPGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-SCW-HHL

   Plasmid

   #190634

   Purpose

   Mammalian expression of genetically encoded calcium indicator SCW-HHL

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCW-HHL
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-β-arrestin2 RRK/Q

   Plasmid

   #38261

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Arg233, Arg237, and Lys251 converted to Glutamines

   Promoter

   CMV

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. RGS-6xHis-mRFP-pcDNA3.1-

    Plasmid

    #52516

    Purpose

    expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    mRFP
    

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    engineered variant of tetrameric red fluorescent …

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIBN-EYFP

    Plasmid

    #103815

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMY-MCS-P2A-BlastR-DYK

    Plasmid

    #163357

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twitch-3 pRSETB

    Plasmid

    #48204

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3A
    

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBAD-SKW-HHL

    Plasmid

    #190632

    Purpose

    Bacterial expression of genetically encoded potassium indicator SKW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SKW-HHL
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIBN-mCherry

    Plasmid

    #103816

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM60

    Plasmid

    #154159

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    4 wt NF-κB sites were replaced with Rel1A-specifi…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLNCX2-Orai1

    Plasmid

    #89818

    Purpose

    Express Orai1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-BICD2N

    Plasmid

    #64070

    Purpose

    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    BICD2N (1-400) (Bicd2 Mouse)
    

    Tags

    GFP and His-ZZ-TCS

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPS0380

    Plasmid

    #133230

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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