163,606 results

1. TFORF2494

   Plasmid

   #142112

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB18 (ZBTB18 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLminP_Luc2P_RE1

   Plasmid

   #90335

   Purpose

   NFkB - p50/p65 pathway gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFKB

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRB-mCherry-Giantin

   Plasmid

   #186575

   Purpose

   Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PMP34-mCherry-EGFP-Tet-On

   Plasmid

   #109017

   Purpose

   Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Fission Yeast, Synthetic)
   naa15 (naa15 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK2014-AaCas12b

   Plasmid

   #121949

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b
   

   Use

   Tags

   10xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Sec61β

   Plasmid

   #172445

   Purpose

   Expression of ER protein Sec61beta with an N-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-TadCBEd-ivt-template

   Plasmid

   #193843

   Purpose

   Template for in vitro transcription of TadCBEd (with SpCas9)

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SpCas9
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBPLexA::p65Uw

   Plasmid

   #26231

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   LexA::p65 (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPROBE-GT

    Plasmid

    #37814

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TFORF1906

    Plasmid

    #141883

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTN4

    Plasmid

    #174212

    Purpose

    Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Inserts

    AtTIR1(F79G)::mRuby (TIR1 Nematode)
    GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    mRuby

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cbh_v5 AAV-ABE C-terminal

    Plasmid

    #137178

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-ETSSB

    Plasmid

    #165549

    Purpose

    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    Extreme Thermostable Single-Stranded DNA Binding Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. STING-miRFP680

    Plasmid

    #200940

    Purpose

    STING fused with miRFP680 for live cell imaging

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    STING (STING1 Human)
    miRFP680
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. iLID-GFP-GCN4-ppKin14

    Plasmid

    #240420

    Purpose

    -end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    ppKin14Vib
    

    Use

    Tags

    GCN4, GFP, and iLID

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-KIF5A-FRB

    Plasmid

    #240425

    Purpose

    +end oriented kinesin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Vim-mCh-FKBP

    Plasmid

    #240423

    Purpose

    Tagged vimentin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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