161,547 results

1. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2049

   Plasmid

   #143866

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PURA (PURA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NUDT3A

   Plasmid

   #42348

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT3A (NUDT3 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP ZO1

   Plasmid

   #30313

   Depositor

   Alan Fanning

   Article

   Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

   Insert

   Zonula Occludens-1 (TJP1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Unique spliceform*

   Promoter

   Available since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_KLF7_P2A_Hygro_Barcode

   Plasmid

   #120493

   Purpose

   Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-EGFP (BPK1098)

   Plasmid

   #133962

   Purpose

   CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

   Depositor

   Benjamin Kleinstiver

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   Promoter

   pPEBV

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro CAG FUCCI

   Plasmid

   #136934

   Purpose

   donor plasmid for constitutive FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
   

   Use

   Synthetic Biology

   Tags

   Clover and mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pemiRFP670-N1

   Plasmid

   #136556

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-EGR1-P2A-mCherry

    Plasmid

    #203856

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLKO.1-UPF1-CDS

    Plasmid

    #136037

    Purpose

    UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1177-X1-8

    Plasmid

    #38150

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2011 May;77(1):53-61

    Insert

    Carboxypeptidase A
    

    Use

    Tags

    HHHHHHRR

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0079 RESCUE-S

    Plasmid

    #130662

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid

    #135002

    Purpose

    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-

    Insert

    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.983G>T, p.G328V

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-ZoAl-RTD-3xF-ORF

    Plasmid

    #238486

    Purpose

    For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD644/645->AA

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-ZoAl-END-3xF-ORF

    Plasmid

    #238485

    Purpose

    For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD1041/1054->AA

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-ZoAl-ENT-3xF-ORF

    Plasmid

    #238484

    Purpose

    For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    R1103A

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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