We narrowed to 14,493 results for: NTS

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pGWB426

Plasmid

#74820

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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FusionRed-PMP-N-10

Plasmid

#56131

Purpose

Localization: Peroxisomes, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

PMP (PXMP2 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-3xFLAG-Puro PAK5 E144K

Plasmid

#167193

Purpose

puromycin resistant lentiviral vector for expression of PAK5 E144K

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 E144K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 M173I

Plasmid

#167192

Purpose

puromycin resistant lentiviral vector for expression of PAK5 M173I

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 M173I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf221

Plasmid

#12909

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf221

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00409

Plasmid

#34517

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5249 (Blimp-1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
prNFL-L269P-EGFP

Plasmid

#132601

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

806T>C, 807G>C; Leu269Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P441L-EGFP

Plasmid

#132604

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1322C>T, 1323A>G; Pro441Leu

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB422

Plasmid

#74816

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB428

Plasmid

#74822

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTaCas9-H840A_6

Plasmid

#91173

Purpose

Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-H840A_1

Plasmid

#91162

Purpose

Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00671

Plasmid

#39722

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6312 (Rfx Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00340

Plasmid

#34463

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8573 (su(Hw) Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0802

Plasmid

#91023

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV

Plasmid

#136380

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTX208

Plasmid

#89268

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01 and OsYSA-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3xFlag-S242R-MEFV

Plasmid

#167020

Purpose

Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Mutation

serine 242 in arginine (S242R)

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-RELΔ424-490

Plasmid

#27267

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Insert

REL (REL Human)

Expression

Mammalian

Mutation

Deletion of transactivation domain I (aa 425-490)

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pAtCas9-dead_1

Plasmid

#91165

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-138-2

Plasmid

#46677

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-138-2 (MIR138-2 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Ares-Aphrodite

Plasmid

#13571

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and Ares

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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