We narrowed to 18,759 results for: nls
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Plasmid#183098PurposepHR-CAG-sfGFP* ABE_3-WPREDepositorInsertGFP*
UseCRISPR and LentiviralAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11429
Plasmid#183205PurposepHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #1
Plasmid#179454PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #2
Plasmid#179455PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 PCNA GFP
Plasmid#105937Purposein vitro transcription, cell cycle nuclear markerDepositorAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCrisprV2 sgRNA hPER2c-term #3
Plasmid#179456PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-p300 HAT
Plasmid#179544Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Blasto
Plasmid#167856PurposePiggyBac compatible plasmid expressing spCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT3L-dCas9
Plasmid#126587PurposeDNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-DNMT3L-dCas9-NLSExpressionMammalianAvailable sinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-NS1-dTomato
Plasmid#175278PurposeAAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomatoDepositorInsertNS1; dTomato (POLY Yellow fever virus strain 17D)
UseAAVTagsdTomato (from bidirectional promoter)Promoterbidirectional TRE promoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2T-CMV-HNHx-ABE8eHD-BlastR
Plasmid#176478PurposeA-to-G base editor with alternative targeting scopeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHNHx-ABE8eHD
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-evoA1-seBE4max-IRES
Plasmid#174699PurposeSmall-molecule controlled base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoA1-seBE4max-IRES
Tagsmyc tagExpressionMammalianMutationUGI-E11KPromoterCMVAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-FlpO
Plasmid#172378PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + FlpO
UseNeurotropic virusAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CVS-N2c-nl.EGFP-SypGFP
Plasmid#172380PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + SypEGFP
UseNeurotropic virusAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CVS-N2c-tdTomato-iCre
Plasmid#172374PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + iCre
UseNeurotropic virusAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)
Plasmid#160645PurposeModule for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLLDepositorInsertP35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
UseSynthetic BiologyExpressionPlantMutationBsmBI sites removedPromoter35S x2Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pUbi-MAD7
Plasmid#170133PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRExpressionPlantPromotermaize Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-WW-CIB1-biLINuS
Plasmid#171757PurposeLentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuSDepositorInsertSynthetic gene containing LexA-WW-CIB1-biLINuS
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
ExpressionMammalianAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti TMEM30A
Plasmid#171789Purposetransfer plasmid for lentiviral vector production expressing TMEM30ADepositorAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNA0306
Plasmid#169452PurposepRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmidDepositorInsertCas9
ExpressionYeastPromoterTEF1pAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTalpha1-iCre
Plasmid#133924PurposeNeuron-specific expression of iCreDepositorInsertiCre
ExpressionMammalianMutationCodon optimizedPromoterneuron-specific tubulin-alpha-1 promoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTalpha1-Dre
Plasmid#133925PurposeNeuron-specific expression of DreDepositorInsertHA-Dre
TagsHAExpressionMammalianPromoterCAG promoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCRISPR V2 sgIREB2_2
Plasmid#169886PurposeDisrupt IREB2DepositorInsertsgRNA targeting IREB2
UseCRISPR and LentiviralExpressionMammalianAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-WT
Plasmid#133751PurposeWild-type Numb tagged with GFP cloned into PWPXLDDepositorAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SlugABEmax
Plasmid#163798PurposeExpresses ABEmax and nSlugCas9DepositorTypeEmpty backboneUseCRISPRExpressionMammalianMutationSlugCas9 (D10A)Available sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-Tetra-com-vector
Plasmid#132553PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the TetraloopDepositorInsertsCas9
sgRNA scaffold with com replacing the Tetraloop
ExpressionMammalianAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
TagsT2A GFPExpressionInsectAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 p300 gRNA
Plasmid#165591PurposeInsertion of p300 degronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP300 (Smarca2 Mouse)
ExpressionMammalianMutationNAAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgGLS_2
Plasmid#163459Purposelentiviral vector expressing Cas9 and an sgRNA targeting GLSDepositorAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgGLS_6
Plasmid#163460Purposelentiviral vector expressing Cas9 and an sgRNA targeting GLSDepositorAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only