We narrowed to 89,698 results for: MAL

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SP-dCas9-VPR

Plasmid

#63798

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Plasmid

#64324

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-WT

Plasmid

#120727

Purpose

Expression of a Flag-tagged version of DDX11 WT in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 (DDX11 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HNF1A_P2A_Hygro_Barcode

Plasmid

#120448

Purpose

Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKIF1C-GFP

Plasmid

#130977

Purpose

Expression of KIF1C-GFP in mammalian cells.

Depositor

Anne Straube

Article

Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

Insert

KIF1C-GFP (KIF1C Human)

Tags

GFP

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mIDUA)

Plasmid

#170123

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Idua Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PX459v3

Plasmid

#178799

Purpose

SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH1-FLAG

Plasmid

#66802

Purpose

Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-TdT

Plasmid

#126424

Purpose

Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Promoter

pCMV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HNF4A_P2A_Hygro_Barcode

Plasmid

#120450

Purpose

Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF4A (HNF4A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEF6-Lck-TurboID

Plasmid

#159433

Purpose

expresses Lck-TurboID in mammalian cells

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Inserts

Lck (LCK Human)
TurboID (BirA mutant)

Tags

HA

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

EF-1a

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH2-R172K-FLAG

Plasmid

#66807

Purpose

Tet-inducible expression of mutant IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH2-FLAG

Plasmid

#66806

Purpose

Tet-inducible expression of IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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